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Publication Open Access Exploring breast cancer data to find the risk factors for distant metastasis : register data, the national cancer institute of Thailand(2552) Auayporn, M; Montida Auayporn; Rojanavipart, P; Piangchan Rojanavipart; Viwatwongkasem, C; Chukiat Viwatwongkasem; Hancharoen, K; Kitiphong Hancharoen; Chaiwerawattana, A; Hancharoen, K; Kitiphong Hancharoen; มนต์ธิดา อวยพร; เพียงจันทร์ โรจนวิภาต; ชูเกียรติ วิวัฒน์วงศ์เกษม; กิติพงษ์ หาญเจริญA retrospective cohort of registered breast cancer patients at the National Cancer Institute of Thailand in 2004 was investigated to explore the association of prognostic factors with the probability of distant metastasis. The analysis was done... using binary and ordinal logistic regression models. There were 893 breast cancer patients included in a 3 year follow up study. During the study period 129(14.45%) were reported as metastasis cases, which the highest number of metastasis patientsPublication Open Access Impact of chest wall motion caused by respiration in adjuvant radiotherapy for postoperative breast cancer patients(2016) C. Lowanichkiattikul; M. Dhanachai; C. Sitathanee; S. Khachonkham; P. Khaothong; Mahidol University. Faculty of Medicine, Ramathibodi Hospitalradiotherapy treatment planning during December 2012 to November 2013 were included. Before scanning the radio-opaque wire was placed on the surface for breast and chest wall visualization on CT images, then the patient underwent three phases of CT scanning...To determine the chest wall movement of each patient during deep inspiratory breath hold (DIBH) and expiratory breath hold (EBH) in postoperative breast cancer patients. Postoperative breast cancer patients who underwent CT simulation for 3DPublication Open Access Determination of whole transcription profiles and specific pathways in invasive ductal breast carcinoma(2013-05-15) Pasra Arnutti; ภัสรา อาณัติ; Manas Kotepui; มนัส โคตรพุ้ย; Wichitra Asanprakit; Phaibul Punyarit; Porntip Chavalitshewinkoon-Petmitr; พรทิพย์ ชวลิตรชีวินกุล เพ็ชรมิตร; Talabporn Harnroongroj; ตรับพร หาญรุ่งโรจน์; Songsak Petmitr; ทรงศักดิ์ เพ็ชรมิตร; Songsak Petmitr; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Nutrition & Food Science.; Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine & Genetics.; Mahidol University. Faculty of Tropical Medicine. Department of Protozoology.Breast cancer is the most common cancer affecting women worldwide including Thailand. Whole transcription profiles of invasive ductal breast carcinoma (IDC) obtained by oligonucleotide microarray should lead to a better understanding... of the molecular basis of IDCs, allow for examination of specific markers for diagnosis, and provide novel targets for therapy. This study aimed to detect the whole transcript expression of approximately 35,000 target genes in Thai breast cancer patients, usingPublication Open Access Cancer-associated fibroblasts induce high mobility group box 1 and contribute to resistance to doxorubicin in breast cancer cells(2014) Kamolporn Amornsupak; Tonkla Insawang; Peti Thuwajit; Pornchai O-Charoenrat; Eccles, Suzanne A.; Chanitra Thuwajit; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of ImmunologyBackground: Cancer-associated fibroblasts and high mobility group box 1 (HMGB1) protein have been suggested to mediate cancer progression and chemotherapy resistance. The role of such fibroblasts in HMGB1 production in breast cancer is unclear.... This study aimed to investigate the effects of cancer-associated fibroblasts on HMGB1 expression in breast cancer cells and its role in chemotherapeutic response. Methods: Breast cancer-associated fibroblasts (BCFs) and non-tumor-associated fibroblastsPublication Open Access ตัวรับฮอร์โมนเอสโตรเจนและโปรเจสเตอโรนในเนื้อเยื่อเต้านมสุนัข(2552) สุกัญญา มณีอินทร์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์ตัวรับฮอร์โมนเอสโตรเจน และตัวรับฮอร์โมนโปรเจสเตอโรน พบได้ในเนื้อเยื่องอกเต้านม การแสดงออกของตัวรับฮอร์โมนทั้งสองชนิดอยู่ภายใต้อิทธิพลของฮอร์โมนเอสโตรเจน และโปรเจสเตอโรน โดยฮอร์โมนเอสโตรเจนทำหน้าที่เพิ่มการแสดงออกของตัวรับฮอร์โมนเอสโตรเจนชนิดอัลฟ่าและตัวรับฮอร์โมนโปรเจสเตอโรน ส่วนฮอร์โมนโปรเจสเตอโรนทำหน้าที่ลดการแสดงออกของตัวรับฮอร์โมนทั้งสองชนิด รูปแบบการแสดงออกของตัวรับฮอร์โมนเอสโตรเจนและโปรเจสเตอโรนในเนื้องอกเต้านมของสุนัข ยังมีความแตกต่างกันและไม่สามารถนำมาใช้ในการพยากรณ์โรคได้ อย่างไรก็ตาม การใช้ฮอร์โมนอาจจะเป็นประโยชน์ในการรักษาเนื้องอกเต้านมที่มีการแสดงออกของตัวรับฮอร์โมนPublication Open Access Effects of alcoholic extract from pomegranate (Punica granatum L.) peels on gastrointestinal nematode egg counts in doe(2013) Sookruetai Boonmasawai; Sivapong Sungpradit; Charoonluk Jirapattharasate; Chowalit Nakthong; Lak Piasai; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Science; Mahidol University. Faculty of Veterinay Science. Department of Clinical Sciences and Public HealthAnthelmintic drug resistant is one of the major problem that quietly reflecting the small ruminant production qualities. Because this problem recently spread out in many areas all over the world, the alternative anthelmintic methods are recently requiring, especially using local economic plants or remedies for reducing the impact. The pomegranate (Punica granatum L.) is one kind of fruit that is used as an anthelmintic plant in Ayurveda remedies for a long time. We investigated the anthelmintic activity of alcoholic extract from pomegranate peels against female goat gastrointestinal nematodes and the toxic effects after treatments. The results were shown that albendazole and pomegranate peel powder could not affect percent of strongyle egg per gram (EPG) when comparing with pre-treatment control data. In contrast, ivermectin injections could significantly affect a first 24h (32 ± 5%), and had continuously effect at day 3, 7 and 14 (31 ± 17%, 51 ± 13% and 33 ± 11%, respectively). Alcoholic extract of pomegranate represented significantly anthelmintic effects at day 1, 3 and 7 (45 ± 11%, 57 ± 14% and 64 ± 7%, respectively) with non-significantly different when comparing with ivermectin. We also preliminary investigated the effects of alcoholic extract from mangosteen (Garcinia mangostana) peels, it could not effect on egg count number per gram when comparing with pre-treatment control. The results suggested that alcoholic crude extracts from pomegranate peels could inhibit gastrointestinal strongyle-type egg production and may be promising used as an alternative anthelmintic drugs in small ruminant farms.Publication Open Access Ethnoveterinary medicine based on Ayurveda plants(2012) Sookruetai Boonmasawai; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal ScienceTraditional plants used for medicine exist for several thousand years ago in India to solve human health problems. The Ayurveda concept involved in the balancing of five basic elements including earth, water, fire, air, and space (ether) because of various active ingredients in remedies. Nowadays, traditional plant uses in veterinary medicine in many regions have been documented and reported such as in Spain, Italy, Argentina, Brazil and Kenya. Catharanthus roseus (Apocynaceae) is an important medicinal plant containing vincristine used in veterinary medicine for treatment of canine transmissible venereal tumor and canine lymphoma. Additional evidences in China showed that Salacia oblonga root in Ayurvedic recipe was able to ameliorate hypertriglyceridemia and excessive ectopic fat accumulation in laying hens. Moreover, crude extract from Terminalia arjuna L. (Combretaceae) bark decreased blood pressure in anaesthetized dogs. However, World Health Organization (WHO) recently focuses in standardization, safety and efficacy of Ayurveda medicine to establish this herbal medicine system. Ayurveda in veterinary medicine still need further clinical research to provide more clear, comprehensive, and practical pharmacological information to improve constantly therapeutic qualities.Publication Open Access Quinoline-based clioquinol and nitroxoline exhibit anticancer activity inducing FoxM1 inhibition in cholangiocarcinoma cells(2015-04-08) Waraporn Chan-on; Nguyen Thi Bich Huyen; Napat Songtawee; Wilasinee Suwanjang; Supaluk Prachayasittikul; Virapong Prachayasittikul; Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied Technology; Mahidol University. Faculty of Medical Technology. Center of Data Mining and Biomedical InformaticsPurpose: Fork head box M1 (FoxM1) is an oncogenic transcription factor frequently elevated in numerous cancers, including cholangiocarcinoma (CCA). A growing body of evidence documents its diverse functions contributing to tumorigenesis and cancer progression. As such, discovery of agents that can target FoxM1 would be valuable for the treatment of CCA. The quinoline-based compounds, namely clioquinol (CQ) and nitroxoline (NQ), represent a new class of anticancer drug. However, their efficacy and underlying mechanisms have not been elucidated in CCA. In this study, anticancer activities and inhibitory effects of CQ and NQ on FoxM1 signaling were explored using CCA cells. Methods: The effects of CQ and NQ on cell viability and proliferation were evaluated using the colorimetric 3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-(4-sulfophenyl)-2H-tetrazolium (MTS assay). Colony formation and cell migration affected by CQ and NQ were investigated using a clonogenic and a wound healing assay, respectively. To demonstrate the agents’ effects on FoxM1 signaling, expression levels of the target genes were quantitatively determined using real-time polymerase chain reaction. Results: CQ and NQ significantly inhibited cell survival of HuCCT1 and Huh28 in a dose- and a time-dependent fashion. Further investigations using the rapidly proliferating HuCCT1 cells revealed significant suppression of cell proliferation and colony formation induced by low doses of the compounds. Treatment of CQ and NQ repressed expression of cyclin D1 but enhanced expression of p21. Most importantly, upon CQ and NQ treatment, expression of oncogenic FoxM1 was markedly decreased concomitant with downregulation of various FoxM1’s downstream targets including cdc25b, CENP-B, and survivin. In addition, the compounds distinctly impaired HuCCT1 migration as well as inhibited expression of matrix metalloproteinase (MMP)-2 and MMP-9. Conclusion: Collectively, this study reports for the first time the anticancer effects of CQ and NQ against CCA cells, and highlights new insights into the mechanism of actions of the quinoline-based compounds to disrupt FoxM1 signaling.Publication Open Access Neutralizing dengue antibody in pregnant Thai women and cord blood.(2015-02-06) Kriangsak Khamim; เกรียงศักดิ์ คำอิ่ม; Weerawan Hattasingh; วีรวรรณ หัตถสิงห์; Ananda Nisalak; Jaranit Kaewkungwal; จรณิต แก้วกังวาล; Fernandez, Stefan; Butsaya Thaisomboonsuk; บุษยา ไทยสมบูรณ์สุข; Krisana Pengsaa; กฤษณา เพ็งสา; Usa Thisyakorn; อุษา ทิสยากร; Weerawan Hattasingh; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pediatrics.; Mahidol University. Faculty of Tropical Medicine. Centre of Excellence for Biomedical & Public Health Informatics (BIOPHICS); Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene.BACKGROUND: The WHO 'Global Strategy for Dengue Prevention and Control, 2012-2020' addresses the growing need for the treatment of dengue, and targets a 25% reduction in morbidity and 50% in mortality (using 2010 estimates as baseline). Achieving these goals requires future dengue prevention strategies that will employ both potential vaccines and sustainable vector-control measures. Maternally transferred dengue antibody is an important factor in determining the optimal age for dengue vaccination. OBJECTIVES: To estimate the seroprevalence of dengue antibodies among mothers living in an area of high endemicity--Ban Pong, Ratchaburi Province--and to assess maternal dengue antibodies transferred to cord blood. MATERIALS & METHODS: A cross-sectional study was conducted with 141 pregnant women who delivered at Ban Pong Hospital, Ratchaburi, Thailand. Maternal-cord paired sera were tested for dengue neutralizing (NT) antibody by PRNT50 assay. A ratio of ≥ 1:10 NT titer to dengue serotype was considered seropositive. RESULTS: Most mothers (137/141, 97.2%) had NT antibodies to at least one dengue serotype in their sera. At birth, the proportion of cord sera with NT antibodies to DEN-1, DEN-2, DEN-3, and DEN-4, were high and similar to the sera of their mothers, at 93.6%, 97.2%, 97.9%, and 92.2%, respectively. The dengue geometric mean titers (GMT) in cord blood were significantly higher than the maternal antibodies (p<0.001): highest in DEN-2, followed by DEN-3, and then DEN-1. The GMT of DEN-4 was the lowest among all four serotypes. CONCLUSIONS: Dengue infection is highly prevalent among pregnant women in this dengue-endemic area. Most of the cord blood had transferred dengue antibodies, which may have an impact on the disease burden in this population.Publication Open Access Retinol-binding protein 4 and its potential roles in hypercholesterolemia revealed by proteomics(2015-08-28) Watcharapong Jugnam-Ang; Supitcha Pannengpetch; Patcharee sarankura-Na-Ayudhya; Chadinee Thippakorn; Chartchalerm Isarankura-Na-Ayudhya; Ratana Lawung; Virapong Prachayasittikul; Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied Technology; Mahidol University. Faculty of Medical Technology. Center for Research and InnovationEffects of hypercholesterolemia on alterations of serum proteins have not been fully elucidated. Herein, using two-dimensional gel electrophoresis (2-DE) in conjunction with LC-MS searching has successfully been carried out to investigate the change of protein expression profiles as consequences of raised blood cholesterol at different levels (normal group: total cholesterol <= 200 mg/dL; borderline high group: total cholesterol 200-239 mg/dL; and high group: total cholesterol ≥ 240 mg/dL) (n = 45). Results revealed that down-regulation of retinol-binding protein 4 (RBP4) (-2.26 fold), transthyretin (-1.25 fold) and gelsolin (-1.47 fold) was observed in the high group. Meanwhile, the other proteins such as haptoglobin, complement factor B and CD5 antigen-like protein were upregulated upto +3.24, +1.96 and +2.04 fold, respectively. Confirmation by Western blotting revealed a significant reduction of RBP4 (approximately 50%) in individual samples derived from the high group. Presumptive conclusion can be drawn that down-regulation of RBP4 might be attributable to the inflammation of adipocytes caused by the release of proinflammatory cytokines (e.g. tumor necrosis factor α and interleukin-1β) from adipose tissues. Moreover, the decrease of transthyretin might also be taken into accounts since it is known that the transthyretin usually forms complex with RBP4 to prevent glomerular filtration and excretion through the kidney. The suppressing effect on RBP4 should be potentiated by the increase of complement factor B and CD5 antigen-like protein, which rendered the adipose tissues to overwhelm the liberation of RBP4 to blood circulation by metabolic and inflammatory processes. Such inflammation could further modulate the induction of cytokine release (e.g. IL-6 and IL-1β), resulting in the synthesis of acute phase protein, in particular, haptoglobin and Creactive proteins from hepatocytes. However, the mechanism of gelsolin reduction remains unclear. Among these differentially expressed proteins, the RBP4 has been proposed as a major linkage between hypercholesterolemia, adipose tissues, liver and kidney, which is believed to be a potential biomarker for metabolic and cardiovascular disorders associated with dyslipidemia in the future. © 2015, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved.