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Publication Open Access In vitro antimicrobial effects of mangosteen extract on peri-implantitis microflora in craniofacial implants.(2013-09) Shrestha, Binit; Sroisiri Thaweboon; สร้อยศิริ ทวีบูรณ์; Suwan Choonharuangdej; สุวรรณ ชุณหเรืองเดช; Boonyanit Thaweboon; บุญนิตย์ ทวีบูรณ์; Theerathavaj Srithavaj; ธีรธวัช ศรีธวัช; Sita Thaworanunta; สิตา ถาวรนันท์; Boonyanit Thaweboon; บุญนิตย์ ทวีบูรณ์; Mahidol University. Faculty of Dentistry. Maxillofacial Prosthetic Service; Mahidol University. Faculty of Dentistry. Department of Oral Microbiologythe antimicrobial effects of mangosteen pericarp extract on peri-implantitis microflora found around craniofacial implants. Material and Methods: The mangosteen pericarp extract was tested against peri-implantitis microflora reference strains of Staphylococcus... aureus ATCC6538, Candida albicans ATCC10231 and clinical strains of Staphylococcus aureus and Candida parapsilosis by disk diffusion test. Minimum inhibitory concentrations (MIC) and minimum cidal concentrations (MCC) were determined using modifiedPublication Open Access Development of cereal and legume based food products for the elderly(2014) Pruet Satusap; Visith Chavasit; Wantanee Kriengsinyos; Kunchit Judprasong; Mahidol University. Faculty of Nutritionand free sugar were criteria for the formulation. Carbohydrate sources were rice flour, brown rice flour, mung bean starch, which carbohydrate in rice flour was the most digestible on in vitro test. Protein and fat sources were soybean flour, black sesame....3) had balanced energy distribution, good quality protein, and energy from saturated fat < 8 kcal/100 kcal and free sugar < 10 kcal/100 kcal. Results from sensory central location test in 219 elderly subjects indicated that the flake snacks from bothPublication Open Access Laboratory Detection of Artemisinin-Resistant Plasmodium falciparum.(2014-06) Kesinee Chotivanich; เกศินี โชติวานิช; Tripura, Rupam; Das, Debashish; Poravuth Yi,c; Day, Nicholas P. J.; Sasithon Pukrittayakamee; ศศิธร ผู้กฤตยาคามี; Chuor, Char Meng; Socheat, Duong; Dondorp, Arjen M.; White, Nicholas J.; White, Nicholas J.; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine.; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford University Tropical Medicine Research Unit.Conventional 48-h in vitro susceptibility tests have low sensitivity in identifying artemisinin-resistant Plasmodium falciparum, defined phenotypically by low in vivo parasite clearance rates. We hypothesized originally that this discrepancy... was explained by a loss of ring-stage susceptibility and so developed a simple field-adapted 24-h trophozoite maturation inhibition (TMI) assay focusing on the ring stage and compared it to the standard 48-h schizont maturation inhibition (WHO) test. In PailinPublication Open Access Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax(2015) Wanlapa Roobsoong; Tharinjaroen, Chayada S; Nattawan Rachaphaew; Porpimon Chobson; Louis Schofield; Liwang Cui; Adams, John H; Jetsumon Sattabongkot; Mahidol University. Faculty of Tropical Medicine. Mahidol Vivax Research Unitin order to improve the culture conditions for P. vivax. Through analysis of the sources of host reticulocytes and nutrients of culture medium, the culture conditions better supporting in vitro growth and maturation of the parasites were identified. Using... this system, three of 30 isolates could be maintained in vitro for over 26 months albeit parasite density is low. Conclusions: Based on the analysis of different culture variables, an improved and feasible protocol for continuous culture of P. vivaxPublication Open Access The effects of serum lipids on the in vitro activity of lumefantrine and atovaquone against Plasmodium falciparum(2012-05-28) Kesinee Chotivanich; เกศินี โชติวานิช; Mathirut Mungthin; มฑิรุทธ มุ่งถิ่น; Ronnatrai Ruengweerayuth; Rachanee Udomsangpetc; รัชนีย์ อุดมแสงเพ็ชร; Dondorp, Arjen M.; Pratap Singhasivanon; ประตาป สิงหศิวานนท์; Sasithon Pukrittayakamee; ศศิธร ผู้กฤตยาคามี; White, Nicholas J.; White, Nicholas J.; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit; Mahidol University. Faculty of Science. Department of Pathobiologyconfounder in laboratory in vitro testing and it could have therapeutic relevance.... of triglyceride-rich plasma lipoproteins. In this study, the consequences of lipidaemia on anti-malarial drug efficacy were assessed in vitro. METHODS: Serum was obtained from non-immune volunteers under fasting conditions and after ingestion of a high fat mealPublication Open Access Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays.(2016-01) Suwanna Korsuwannawong; สุวรรณา ก่อสุวรรณวงศ์; La‑Ongthong Vajrabhaya; ละอองทอง วัชราภัย; Suwanna Korsuwannawong; Mahidol University. Faculty of Dentistry. Research OfficeObjectives: The aim of this study was to compare the results of dimethylthiazol diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays of Clinacanthus nutans cytotoxicity. Materials and Methods: Mouse fibroblast (L929) cells were exposed to 0.01%, 0.1%, 0.25%, and 0.5% (W/V) C. nutans in a 96-cluster -well-culture plate for 24 h. The cell viability after exposure to C. nutans was determined by MTT and NRU assays in separate tissue culture plates. The two assays were compared through an intra‑class correlation coefficient (ICC) analysis. Results: No significant differences in cytotoxicity were noted between the two assays ( P > 0.05). The ICC values for agreement between two assays for the negative and positive control groups and C. nutans concentrations of 0.01%, 0.1%, 0.25%, and 0.5% were 0.84, 0.83, 0.77, 0.68, 0.74, and 0.71, respectively. Conclusion: In general, the MTT and NRU assays performed similarly, exhibiting moderate to good correlation for the evaluation of the cytotoxicity of C. nutans .Publication Open Access Birth of kittens after the transfer of frozen-thawed embryos produced by intracytoplasmic sperm injection with spermatozoa collected from cryopreserved testicular tissue.(2012) T Tharasanit; S Buarpung; S Manee-In; C Thongkittidilok; N Tiptanavattana; P Comizzoli; M Techakumphu; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public Healthinto in vitro matured cat oocytes. At 24 h post-ICSI, presumptive zygotes/cleaved embryos were treated with 10 μm forskolin for 24 h to reduce intracellular lipid content of embryos (delipidation). At 48 h after oocyte injection, cleaved embryos (2- to 8-cell... stage) were frozen in 10% (v/v) ethylene glycol-based medium by a slow controlled rate method and stored in liquid nitrogen. To evaluate in vitro and in vivo developmental competence, frozen embryos were thawed and then cultured for 6 days (n = 155Publication Open Access The effect of zirconia framework design on the failure of all-ceramic crown under static loading.(2015-04-23) Somchai Urapepon; สมชาย อุรพีพล; Pakamard Taenguthai; ผกามาศ แตงอุทัย; Somchai Urapepon; Mahidol University. Faculty of Dentistry. Prosthodontics DepartmentPURPOSE: This in vitro study aimed to compare the failure load and failure characteristics of two different zirconia framework designs of premolar crowns when subjected to static loading. MATERIALS AND METHODS: Two types of zirconia frameworks... at the lingual incline of the buccal cusp until fracture using a universal testing machine with cross-head speed 0.5 mm/min. The load to fracture values (N) was recorded and statistically analyzed by independent sample t-test. RESULTS: The mean and standardPublication Open Access Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum.(2010-11) Preeyaporn Monatrakul; ปรียาภรณ์ โมนะตระกูล; Mathirut Mungthin; Dondorp, Arjen M.; Srivicha Krudsood; ศรีวิชา ครุฑสูตร; Rachanee Udomsangpetch; Polrat Wilairatana; พลรัตน์ วิไลรัตน์; White, Nicholas J.; Kesinee Chotivanich; เกศินี โชติวานิช; Kesinee Chotivanich; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit.efficacy of anti-malarial drugs, but the mechanism and magnitude of this effect has not been characterized. This study characterized the effects of 'immune' plasma to Plasmodium falciparumon the in vitro susceptibility of P. falciparum to anti-malarial... and its effects on in vitro parasite growth and multiplication of the Thai P. falciparum laboratory strain TM267 were assessed by light microscopy. The in vitro susceptibility to quinine and artesunate was then determined in the presence and absencePublication Open Access Shear bond strength of Zirconia to different adhesive resin cements.(2015-05) Porntida Visuttiwattanakorn; พรธิดา วิสุทธิวัฒนากร; Noi Rithy; Kallaya Suputtamonkol; กัลยา ศุพุทธมงคล; Widchaya Kanchanavasita; วิชญ กาญจนะวสิต; Mahidol University. Faculty of Dentistry. Prosthodontic DepartmentObjectives: This in-vitro study investigated the shear bond strengths(SBS) and failure modes of 2 resin cements bonded to zirconia core ceramic. Materials and methods: Forty square-shaped (8 mm x 8 mm x 2 mm) and cylinder-shaped (5 mm diameter... of the square and cylinder-shaped blocks were sandblasted with 50 μm Al2O3 at 2-bar pressure. After bonding, specimens were stored in distilled water at 37 ˚C for 24 hrs and then shear bond test was performed using a universal testing machine. The bond