191 results
Search Results
Now showing 1 - 10 of 191
Publication Metadata only Determination of tafenoquine in dried blood spots and plasma using LC and fluorescence detection(2011-08-01) Susanne Römsing; Niklas Lindegardh; Yngve Bergqvist; Hogskolan Dalarna; Uppsala Universitet; Center for Clinical Research Dalarna; Mahidol University; Nuffield Department of Clinical Medicineof the method was 50 nmol/l. Conclusion: The developed method has adequate sensitivity and is highly suitable for clinical studies in dried blood spots and plasma. © 2011 Future Science Ltd..... Results: A bioanalytical method for the determination of tafenoquine in 100 l of capillary blood applied onto sampling paper and in 100 l of plasma has been developed and validated. The Whatman 31 ET Chr paper was treated with 0.6 mol/l tartaric acidPublication Metadata only Development and validation of an automated solid-phase extraction and liquid chromatographic method for determination of lumefantrine in capillary blood on sampling paper(2007-10-18) D. Blessborn; S. Römsing; A. Annerberg; D. Sundquist; A. Björkman; N. Lindegardh; Y. Bergqvist; Hogskolan Dalarna; Uppsala Universitet; Center for Clinical Research Dalarna; Mahidol University; Nuffield Department of Clinical Medicine; Karolinska InstitutetA bioanalytical method for the determination of lumefantrine in 100 μl blood applied onto sampling paper, by solid-phase extraction and liquid chromatography, has been developed and validated. Whatman 31 ET Chr sampling paper was pre-treated with 0....75 M tartaric acid before sampling capillary blood to enable a high recovery of lumefantrine. Lumefantrine was extracted from the sampling paper, then further purified using solid-phase extraction and finally quantified with HPLC. The between-dayPublication Open Access Effect of sex, age and strain on hematological and blood clinical chemistry in healthy canine(2012) Arunee Jangsangthong; Pongpun Suwanachat; Pariyakorn Jaykum; Supakit Buamas; Waraporn Kaewkongjan; Shutipen Buranasinsup; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Science; Mahidol University. Faculty of Veterinay Science. Laboratory of Prasuarthon hospitalThe hematological and blood clinical chemistry are essential for determine the physical health, diagnosis and prognosis of the disease. However, they may vary with race, nutrition, age, sex, stress and disease. The aim of the present study... was to investigatethe effect of sex, age and strain on the hematology and blood clinical chemistry. Canines (n=134)were divided into 5 groups depend on age, 2 groups depend on sex and 4 groups depend on strain. The effect of age was found onmonocytes, neutrophilsPublication Open Access Effect of sex, age and strain on hematological and blood clinical chemistry in healthy canine(2012) Arunee Jangsangthong; Pongpun Suwanachat; Pariyakorn Jaykum; Supakit Buamas; Waraporn Kaewkongjan; Shutipen Buranasinsup; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Science; Mahidol University. Faculty of Veterinary Science. Laboratory of Prasuarthon hospitalThe hematological and blood clinical chemistry are essential for determine the physical health, diagnosis and prognosis of the disease. However, they may vary with race, nutrition, age, sex, stress and disease. The aim of the present study... was to investigatethe effect of sex, age and strain on the hematology and blood clinical chemistry. Canines (n=134)were divided into 5 groups depend on age, 2 groups depend on sex and 4 groups depend on strain. The effect of age was found onmonocytes, neutrophilsPublication Open Access ค่าทางโลหิตวิทยาและเคมีคลินิกในเลือดแมวที่มีสุขภาพดี(2557) ปริยากร จายคำ; พงษ์พันธุ์ สุวรรณชาติ; ศุภกิจ บัวมาศ; อรุณี แจ้งแสงทอง; วราภรณ์ แก้วคงจันทร์; วชิร ตระกูลชัยศรี; ชุติเพ็ญ บูรณะสินทรัพย์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์Hematology and clinical blood chemistry values are important for evaluate the health of animals, diagnostic and prognosis. Animals that have any health problem will have different in hematological and clinical blood chemistry value from... that of standard values. However, most standard values laboratory in Thailand come from foreign countries. The different in strain (breeds), environmental and food may affect hematology and clinical blood chemistry. To establish the normal hematology and clinicalPublication Metadata only High sensitivity methods to quantify chloroquine and its metabolite in human blood samples using LC-MS/MS(2019-03-01) Karnrawee Kaewkhao; Kesinee Chotivanich; Markus Winterberg; Nicholas Pj Day; Joel Tarning; Daniel Blessborn; Mahidol University; Nuffield Department of Clinical Medicine© 2019 2019 Karnrawee Kaewkhao. Aim: Chloroquine is an antimalarial drug used in the treatment of Plasmodium vivax malaria. Three methods to quantify chloroquine and its metabolite in blood matrices were developed and validated. Methodology... & results: Different high-throughput extraction techniques were used to recover the drugs from whole blood (50 μl), plasma (100 μl) and dried blood spots (15 μl as punched discs) followed by quantification with LC-MS/MS. The intra- and inter-batch precisionsPublication Metadata only Assay for screening for six antimalarial drugs and one metabolite using dried blood spot sampling, sequential extraction and ion-trap detection(2010-11-01) Daniel Blessborn; Susanne Romsing; Yngve Bergqvist; Niklas Lindegardh; Hogskolan Dalarna; Uppsala Universitet; Mahidol University; Center for Clinical Research Dalarna; Nuffield Department of Clinical Medicineto interviewing patients, which will increase reliability of surveys. Results: This assay detects quinine, mefloquine, sulfadoxine, pyrimethamine, lumefantrine, chloroquine and its metabolite desethylchloroquine in a 100-μl dried blood spot. Most of the drugs also... have long half-lives that make them detectable at least 7 days after administration. The drugs are extracted from the dried blood spot with sequential extraction (due to the big differences in physicochemical properties), solid-phase extraction is usedPublication Metadata only A new approach to evaluate stability of amodiaquine and its metabolite in blood and plasma(2006-04-11) D. Blessborn; G. Neamin; Y. Bergqvist; N. Lindegårdh; Hogskolan Dalarna; Mahidol University; Nuffield Department of Clinical MedicineA stability study for amodiaquine (AQ) and desethylamodiaquine (AQm) in whole blood and plasma is reported. AQ, AQm and chloroquine (CQ) were simultaneously analysed and the ratios AQ/CQ and AQm/CQ were used to ensure correct interpretation... of the stability results. CQ was stable in whole blood and plasma at all tested temperatures enabling it to be a stability marker in stability studies. Simultaneous analysis of compounds, of which at least one is already known to be stable, permits a within samplePublication Metadata only Quantification of dihydroartemisinin, artesunate and artemisinin in human blood: Overcoming the technical challenge of protecting the peroxide bridge(2011-07-01) Niklas Lindegardh; Warunee Hanpithakpong; Benjamas Kamanikom; Janhom Pattayaso; Pratap Singhasivanon; Nicholas J. White; Nicholas P J Day; Mahidol University; Nuffield Department of Clinical MedicineBackground: Quantification of artemisinin (ARN) and its derivatives in whole blood has hitherto been thought impossible. Results: A LC-MS/MS method for the analysis of artesunate (ARS), its metabolite dihydroartemisinin (DHA) and artemisinin... in human whole blood has been developed and successfully validated. The method includes stabilization of the blood matrix at the time of collection and at the time of analysis. Addition of potassium dichromate to the blood samples deactivated the Fe 2Publication Metadata only Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds(2013-01-01) Daniel Blessborn; Karl Sköld; David Zeeberg; Karnrawee Kaewkhao; Olof Sköld; Martin Ahnoff; Mahidol University; Nuffield Department of Clinical Medicine; Denator AB; AstraZeneca Sweden; Goteborgs Universitetstabilization by rapid, high-temperature heating was used. The stability of six commercial drugs in blood and blood spots was investigated under various conditions with or without heat stabilization at 95°C. Oseltamivir, cefotaxime and ribavirin were...: Heat stabilization is a viable technique to maintain analytes in blood spot samples, without the use of chemical additives, by stopping the enzymatic activity that causes sample degradation. © 2013 Denator AB.