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Publication Metadata only The fertilization performance in vivo of rabbit spermatozoa capacitated in vitro(1981-01-01) P. Viriyapanich; J. M. Bedford; Weill Cornell Medical College; Mahidol UniversityAlthough mammalian spermatozoa can be capacitated in vitro appropriately for the accomplishment of fertilization in vitro, it has not been established whether such spermatozoa can fertilize immediately in the Fallopian tube nor whether... their fertilizing ability is comparable to that of spermatozoa capacitated in vivo. Rabbit spermatozoa treated here in a way reported to capacitate them for fertilization in vitro (20 min in high ionic strength medium (HIS) of 380 mOsm/kg at 37°C) couldItem Metadata only Knowledge and attitude toward invitro-fertilization among infertile women attending the infertility clinic in Ramathibodi hospital(Mahidol University. Mahidol University Library and Knowledge Center, 2003) Suphamas Partiprajak; Charnchai Suchartwatanachai; Pratak O-Prasertsawat; Yunyong Ampawa; Pansak SugkraroekPublication Metadata only In vitro fertilization of lobster oocytes(1991-01-01) P. Talbot; W. Poolsanguan; B. Poolsanguan; H. Al‐Hajj; University of California, Riverside; Mahidol University; The University of JordanThe feasibility of fertilizing preovulatory lobster oocytes was examined in vitro under various experimental conditions. Large (1.6 mm diameter) and small (0.6 mm diameter) oocytes were compared in fertilization trials. Small oocytes were surrounded... to bind to large diameter oocytes that were induced to ovulate in vitro by collagenase treatment. Contaminating enzymes may have destroyed the sperm receptor in envelope 1A of collagenase treated oocytes. Our in vitro fertilization method will allowPublication Metadata only Effects of piperine on hamster sperm capacitation and fertilization in vitro(1991-01-01) P. PIYACHATURAWAT; W. SRIWATTANA; P. DAMRONGPHOL; C. PHOLPRAMOOL; Department of Physiology; Mahidol UniversityThe effect of piperine on the fertilizing ability of hamster sperm was investigated in vitro. Sperm were incubated in a capacitation medium for 3 h prior to co‐incubation with hamster eggs in a fertilization medium for another 3 h. Addition of 0....18–1.05 mM piperine to the capacitation medium reduced both the percentage of eggs fertilized and the degree of polyspermia in a dose‐dependent manner. When piperine was added to the fertilization medium alone, a significant reduction in fertilizationPublication Metadata only In vitro maturation and fertilization of swamp buffalo oocytes and their subsequent development(1992-01-01) K. Pavasuthipaisit; Y. Kitiyanant; C. Thonabulsombat; C. Tocharus; S. Sriurairatna; K. L. White; Mahidol University; University LoganThe present experiment was carried out to evaluate the maturation, fertilization and subsequent embryo culture of swamp buffalo oocytes in vitro. The oocytes (n=273) were collected and morphologically graded based on the structure of cumulus-oocyte... in the Grade 1 (64%) and Grade 2 (68%) groups. Development to the 6 to 8 cell stage substantiated fertilization of Grade 1 and 2 oocytes. These results demonstrated that swamp buffalo oocytes are capable of maturing in vitro, forming embryos, and developingPublication Metadata only Chromosome analysis of unfertilized oocytes after in vitro fertilization program: a preliminary report.(1995-12-01) O. Makemaharn; S. Srivannaboon; Mahidol UniversityTo assess the contribution of chromosome anomalies to high failure of in vitro fertilization (IVF), a total of sixty three eggs from 37 women participating in the IVF program were cytogenetically investigated. The mean age of the oocytes donors.... It may explain for early pregnancy loss, low pregnancy rate after in vitro fertilization.Publication Metadata only Chromosomal analysis of "failed-fertilized" human oocytes resulting from in-vitro fertilization and intracytoplasmic sperm injection(2001-04-01) Somboon Kunathikom; Singpetch Suksompong; Orawan Makemaharn; Pitak Laokirkkiat; Mahidol UniversityTo evaluate the incidence of chromosomal abnormalities in "failed-fertilized" oocytes derived from in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedure, a cytogenetic analysis was performed on 164 IVF and 64 ICSIItem Metadata only Inhibition of hamster sperm capacitation and fertilization by piperine(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Worapot Sriwattana; Pawinee Piyachaturawat; Chumpol Pholpramool; Praneet DamrongpholThe effect of piperine of fertilizing ability of hamster spermatozoa was investigated in both in vitro and in vivo systems. In the in vitro system, spermatozoa were incubated in capacitation medium which contained various concentrations of pipeine... ability. A marked decrease in per cent motility of spermatozoa from the piperine-treated group (100 mg/kg BW) during incubation in capacitating medium was observed. However, it did not affect fertilization in vitro at this level of drug treatmentPublication Metadata only Development master arm of 2-DOF planar parallel manipulator for In-Vitro Fertilization(2018-02-07) Kitti Thamrongaphichartkul; Supachai Vongbunyong; Lalana Nuntakarn; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; King Mongkut s University of Technology Thonburi© Published under licence by IOP Publishing Ltd. Micromanipulator is a mechanical device used for manipulating miniature objects in the order of micron. It is widely used in In-Vitro Fertilization (IVF) in which sperms will be held in a micro-needle... and penetrate to an oocyte for fertilization. IVF needs to be performed by high skill embryologists to control the movement of the needle accurately due to the lack of tactile perception of the user. Haptic device is a device that can transmit and simulatePublication Metadata only Proportion of sperm and eggs for maximal in vitro fertilization in haliotis asinina and the chronology of early development(2010-11-01) Worawit Suphamungmee; Attakorn Engsusophon; Rapeepun Vanichviriyakit; Prapee Sretarugsa; Jittipan Chavadej; Tanes Poomtong; Vichai Linthong; Prasert Sobhon; Mahidol University; Coastal Aquaculture Research and Development CenterTo obtain the highest yield during in vitro fertilization of tropical abalone Haliotis asinina, optimal proportion of the gametes, the timing of sperm-egg interaction, and subsequent development were investigated. The highest yield of fertilization... and become bound to the vitelline envelope within 30 sec, followed by an acrosomal reaction at 1 min. The fertilized egg extrudes the first and second polar bodies at 810 min, and then the zygote begins cleavage at 1520 min. This is followed by the second