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Item Metadata only Characterization of penicillin G acylase from bacillus subtilis (pBA 401)(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Arunsri Sritanaitipol; Pintip Ruenwongsa; Bhinyo PanijpanPenicillin G acylase (PA) is the enzyme used commercially for the production of semisynthetic penicillins. Penicillin G acylase gene from Bacillus megaterium has been cloned into Bacillus subtilis which can release the enzyme extracellularly.... The enzyme was extracted from the medium by using celite adsorption and eluted with 24% ammonium sulfate. The enzyme was then further purified on FPLC using Mono Q column (HR10/10) and Superose-12 column (S16/50). Two peaks of penicillin G acylase, i.e., peakItem Metadata only Nucleotide sequence analysis and strain improvement for high expression of penicillin G acylase gene from Bacillus megaterium UN-1(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Kanchana Weeradechapon; Watanalai Panbangred; Vithaya Meevootisom; Chuenchit BoonchirdPenicillin G acylase (PAC) catalyzes the hydrolysis of benzylpenicillin to 6-aminoper,icillanic acid (6-APA), a key intermediate, in the synthesis of semisynthetic peniciliins. The penicillin G acylase gene (pac) gene was isolated from Bacillus... megaterium UN-1, a high penicillin G acylase producer mutant of Bacillus megaterium ATCC 14945. Complete nucleotide sequence of pac gene was determined and found that the gene contained an open reading frame encoding for 802 amino acids with MW of about 92Publication Metadata only Phase transfer bioconversion of penicillin G into 6-APA by immobilized penicillin acylase in recycling aqueous two-phase systems with a light-sensitive copolymer(2007-06-01) Ke Ming Jin; Xue Jun Cao; Ying Ping Zhuang; Ju Chu; Si Liang Zhang; Duerasor Orawan; East China University of Science and Technology; Mahidol UniversityImmobilized penicillin acylase was used for bioconversion of penicillin G into 6-APA in aqueous two-phase systems consisted of a light-sensitive polymer (PNBC 300000) and Dextran 20000. Partition coefficients of 6-APA was found to be about 8... that of the control in the reaction of single aqueous phase buffer. Three repeated batches of reactions were carried out. In aqueous two-phase systems, substrate penicillin G, products 6-APA and phenylacetate acid are biased in top phase, while immobilized penicillinItem Metadata only Conversion of bacterial penicillin-G acylase to cephalosporin-C acylase by protein engineering(Mahidol University. Mahidol University Library and Knowledge Center, 2003) Catleya Rojviriya; Jirundon Yuvaniyama; Pimchai Chaiyen; Vithaya MeevootisomItem Metadata only Studies on the purification and characterization of penicillin G acylase from Bacillus megaterium pBA 402/5(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Ampa Sriinkaew; Kavi Ratanabanangkoon; Vithaya MeevootisomPenicillin G acylase is an enzyme which hydrolyzes benzylpenicillin to 6-aminopenicillanic acid, a key intermediate in the manufacturing of semisynthetic penicillins. Purification methods of extracellular penicillin G acylase from B. megaterium p... than 11 . Km and Vmax values of the enzyme were 12.5 mM penicillin G and 16.7 unit/ml, respectively. When 6-nitro-3-phenylacetamidobenzoic acid (NIPAB) was used as a substrate, these values were 0.53 mM and 8.20 unit/ml, respectively. Phenylacetic acidPublication Metadata only Pharmacokinetics of Rheumatic Fever Prophylaxis Regimens(1992-01-01) V. Thamlikitkul; S. Kobwanthanakun; S. Pruksachatvuthi; R. Lertluknithi; Mahidol UniversityThe pharmacokinetics of benzathine penicillin G (1200000 IU given intramuscularly), penicillin V (250 mg given orally twice daily), erythromycin stearate (250 mg given orally twice daily) and roxithromycin (150 mg given orally once daily) were... at intervals of up to 28 days after intramuscular injection and immediately before and 1–2 h after the fifth oral dose. The concentrations of penicillin G in all serum samples obtained on day 28 after parenteral benzathine penicillin G administration werePublication Metadata only Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration(2011-12-01) Catleya Rojviriya; Thunyaluck Pratumrat; Mark A. Saper; Jirundon Yuvaniyama; Mahidol University; University Michigan Ann ArborPenicillin G acylase from Bacillus megaterium (BmPGA) is currently used in the pharmaceutical industry as an alternative to PGA from Escherichia coli (EcPGA) for the hydrolysis of penicillin G to produce 6-aminopenicillanic acid (6-APA), a penam... nucleus for semisynthetic penicillins. Despite the significant differences in amino-acid sequence between PGAs from Gram-positive and Gram-negative bacteria, a representative PGA structure of Gram-positive origin has never been reported. In this studyPublication Metadata only An open, randomized, controlled trial of penicillin, doxycycline, and cefotaxime for patients with severe leptospirosis(2004-11-15) Yupin Suputtamongkol; Kanigar Niwattayakul; Chuanpit Suttinont; Kitti Losuwanaluk; Roongroeng Limpaiboon; Wirongrong Chierakul; Vanaporn Wuthiekanun; Surapee Triengrim; Mongkol Chenchittikul; Nicholas J. White; Mahidol University; Muang Loei Ram Hospital; Maharaj Nakhon Ratchasima Hospital; Banmai Chaiyapod Hospital; Udon Thani Center Hospital; National Institutes of Health, Bethesda; Churchill Hospital, there is no consensus regarding the optimal treatment for severe leptospirosis. Methods. An open-label, randomized comparison of parenteral cefotaxime, penicillin G sodium (hereafter known as "penicillin G"), and doxycycline for the treatment of suspected severe... leptospirosis. A total of 132 patients had rickettsial infection diagnosed, and, for these patients, treatment with doxycycline was superior to treatment with penicillin G. Conclusions. Doxycycline or cefotaxime is a satisfactory alternative to penicillin GPublication Metadata only High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant(2000-08-15) W. Panbangred; K. Weeradechapon; S. Udomvaraphant; K. Fujiyama; V. Meevootisom; Mahidol University; Osaka UniversityBy marker exchange mutagenesis, Bacillus megaterium strain UN-1 (Bm-UN1) was used to prepare a mutant strain B. megaterium UN-cat (Bm-UNcat) lacking the penicillin G acylase gene (pac). The pac gene from Bm-UN1 was subcloned into pTF6... and the resultant plasmid, pBA402, was introduced into Bm-UNcat and Bacillus subtilis. Bm-UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13.7, 19.5 and 20.4 U ml1 at 24, 36 and 48 h of culture, respectively. This was two- to fivefoldItem Metadata only Whole-genome sequence analysis of high-level penicillin-resistant strains and antimicrobial susceptibility of Neisseria gonorrhoeae clinical isolates from Thailand(2022-07-01) Nokchan N.; Wongsurawat T.; Jenjaroenpun P.; Nitayanon P.; Tribuddharat C.; Mahidol Universityby the agar dilution method. PCR amplification and sequencing of 23S rRNA and mtrR (a negative regulator of MtrCDE efflux pump) were performed. Whole genomes of 3 PPNG strains with high-level penicillin resistance (MIC ≥ 128 μg/ml) were sequenced using... recommended for gonorrhea treatment between 2015 and 2017, and the AMR determinants and genetic compositions of plasmids in 3 gonococcal strains with high-level penicillin resistance. Methods We collected 117 N. gonorrhoeae isolates from patients