Browsing by Author "ภานพ วิไลนาม"

Filter results by typing the first few letters
Now showing 1 - 3 of 3
  • Thumbnail Image
    PublicationOpen Access
    Electrocution death and pathological findings with the metallization in wild elephant: case report
    (2018) Wasinee Thepapichaikul; Panop wilainam; Parin Suwannaprapha; วาสินี เทพอภิชัยกุล; ภานพ วิไลนาม; ปริญ สุวรรณประภา; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. โรงพยาบาลสัตว์และสัตว์ป่าปศุปาลัน; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาปรีคลินิกและสัตวศาสตร์ประยุกต์
    An about 20-year-old, male wild elephant was found dead on the road and electrocution was suspected to be the cause. Electrocution of wild elephants was rarely documented. On necropsy examination, an electrical mark may be the only external appearance with no appearance of specific gross lesions on the internal organs. The pathologist will use evidence from the scene with histopathological investigation to make the diagnosis of the cause of death. The necropsy determined electrical marks on the trunk and legs of the elephant, while hemorrhage and congestion were found in the lungs, heart and omentum. Microscopically, skin showed epidermal separation with streaming cytonuclear elongation. The metallization of iron and copper were presented on the electric skin burn. The myocardium was fragmented and presented as square nuclei. These findings were typical morphologic changes that supported the differential diagnosis of skin lesions and internal organ damage caused by electrocution. This is the first description of a post-mortem with distinctive histopathologic investigation in electrocution of wild elephant.
  • Thumbnail Image
    PublicationOpen Access
    Mast cell activation in the skin of Plasmodium falciparum malaria patients.
    (2015-02-07) Panop Wilainam; ภานพ วิไลนาม; Rungrat Nintasen; Parnpen Viriyavejakul; พรรณเพ็ญ วิริยเวชกุล; Parnpen Viriyavejakul; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology.; Mahidol University. Faculty of Veterinary Science. Department of Preclinic and Applied Animal Science.
    BACKGROUND: Mast cells (MCs) play an important role in the immune response and inflammatory processes. Generally, MCs can be stimulated to degranulate and release histamine upon binding to immunoglobulin E (IgE). In malaria, MCs have been linked to immunoglobulin (Ig) E-anti-malarial antibodies. This study investigated the response of MCs in the skin of patients with Plasmodium falciparum malaria. METHODS: Skin tissue samples were examined from ten uncomplicated and 20 complicated P. falciparum malaria cases. Normal skin tissues from 29 cases served as controls. Pre- and post-treatment tissues were included. Histopathological changes of the skin were evaluated using haematoxylin and eosin stain. MCs were investigated using toluidine blue staining. The percentage of MC degranulation was compared among groups and correlated with clinical data. RESULTS: MC degranulation was significantly higher in the complicated P. falciparum (43.72% ± 1.44) group than the uncomplicated P. falciparum (31.35% ± 3.29) (p <0.05) and control groups (18.38% ± 1.75), (p <0.0001). MC degranulation correlated significantly with the degree of parasitaemia (r s  = 0.66, p <0.0001). Associated pathological features, including extravasation of red blood cells, perivascular oedema and leukocyte infiltration were significantly increased in the malaria groups compared with the control group (all p <0.001). CONCLUSIONS: MCs in the skin dermis are activated during malaria infection, and the degree of MC degranulation correlates with parasitaemia and disease severity.
  • Thumbnail Image
    PublicationOpen Access
    Quantitative evaluation of cellular intensity in cytologic staining over difference time period of post air-dried smear in canine mammary gland tumor
    (2018) Jeerasak sri-in; Pattita Ruayaree; Panpaga Sangsuriya; Panop wilainam; Parin Suwannaprapha; จีรศักดิ์ ศรีอินทร์; ปทิตตา รวยอารี; พรรณพงา เเสงสุริยะ; ภานพ วิไลนาม; ปริญ สุวรรณประภา; จุฬาลงกรณ์มหาวิทยาลัย. คณะเภสัชศาสตร์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาปรีคลินิกและสัตวศาสตร์ประยุกต์
    Diagnostic cytology is an initial laboratory testing to diagnose various types of cancer, infections and inflammatory diseases. The collection and processing of cytological specimens are very important to produce good quality specimens for accurate and reliable cytological interpretations. In this study, 208 cytologic specimens were obtained from 26 dogs that have diagnosed of canine mammary gland tumors. The quantitative of cellular intensity in staining quality was evaluated in varying interval time of post air-dried smear by usage of light microscope and computer software. The results showed that the intensity of cells, nucleus, cytoplasm, and the percentage of neoplastic cells with distinct cell boundaries, which were parameters indicating staining quality, were statistical significant at all period of post air-dried smear when compared to specimens immediately stained (p<0.05). In conclusion, the results were demonstrated that any delay in staining post air-dried smears affected the cellular intensity and consequence adversely affected the quality of cytologic specimens.