Browsing by Author "Nopachai Suthisai"

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    Acetylcholinesterase and cholinesterase activities in giardia lamblia trophozoites cultured in vitro
    (2004) Cheeraratana Cheeramakara; จีระรัตน์ จีระมะกร; Apichart Nontprasert; อภิชาต นนท์ประเสริฐ; Kriyaporn Songmuaeng; กริยาพร สองเมือง; Wanyarat Thanomsak; วัลยารัตน์ ถนอมศักดิ์; Chutatip Siripanth; จุฑาทิพ ศิริพันธุ์; Nopachai Suthisai; นภชัย สุทธิสัย; Charnnarong Sanghirun; ชาญณรงค์ แสงหิรัญ; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Radioisotope; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine; Mahidol University. Faculty of Tropical Medicine. Department of Protozoology
    Axenic culture of Giardia lamblia trophozites in vitro was conducted from patients infected with Giardia lamblia. The cholinesterase (ChE) and acetylcholinesterase (AChE) activities were determined in cultured Giardia trophozoites. The enzyme activities were found in boh tissue-bound and tissue-free forms. There was low ChE and AChE enzyme activities in trophozoites (1.6095 ± 1.0981; 0.9626 ± 0.1322; 0.1387 ± 0.0783; 0.0752 ± 0.0877 Units/ mg protein, respectively). The tissue-bound and tissue-free ChE enzymes were found in a greater amount than tissue-bound and tissue-free AChE enzymes. The presence of these enzymesin Giardia may involved in the motility of Giardia trophozoites.
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    Acetylcholinesterase and cholinesterase activities in rats infected with giardia lamblia
    (2004) Cheeraratana Cheeramakara; จีระรัตน์ จีระมะกร; Apichart Nontprasert; อภิชาต นนท์ประเสริฐ; Wanyarat Thanomsak; วัลยารัตน์ ถนอมศักดิ์; Kriyaporn Songmeang; กริยาพร สองเมือง; Nopachai Suthisai; นภชัย สุทธิสัย; Bangorn Sukchut; Suvit Areekul; สุวิทย์ อารีย์กูล; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Radioisotope; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine
    Thirty-three adult Wistar rats were assigned randomly to receive either 1.0 x 106 human Giardia lamblia cysts (n = 18) or controls (n = 15). Actylcholinesterase (AChE) and cholinesterase (ChE) activities in the infected rats were compared with the controls. The results showed that the plasma ChE activities in the infected rats were statistically significant higher than controls on day 10, 25, 29, 33, and 40 post-infection and decreased to baseline after day 49 post-infection. The red cell AChE activities in the infected rats were higher than the controls on day 37, 55, and 58 post-infection. There was a reverse correlation between Giardia cysts output and red cell AChE activity in the infected rats. This study suggested that there was no liver involvement but enhancement of erythropoiesis secondary to anemia in rats infected with Giardia lamblia.
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    Distribution of C14-labelled arteether in kidneys and livers of experimental mice after intramuscular injection
    (2008-03-01) Cheeraratana Cheeramakara; Vasant Khachonsaksumet; Nopachai Suthisai; Wanyarat Nakosiri; Kriyaporn Songmuaeng; Channarong Saenghirun; Duangduen Phienpicharn; Apichart Nontprasert; Mahidol University
    To study the distribution and localization of oil-soluble arteether in experimental mice, we injected C14-labelled arteether (20 μCi/kg body weight) intramuscularly and measured radioactivity in the blood, kidney, and liver. The labelled arteether distributed and localized more to the kidney (819,180.4 ± 34,134 dpm/cm3) than the liver (288,628.9 ± 54,954 dpm/cm3) 4 hours post-injection. The main localization of labelled arteether was in the kidney cortex rather than the medulla (p < 0.05). However, the distribution of radioactivity was homogeneous in the liver. The terminal half-life of labelled arteether in the blood was 1.8 hours. The blood: kidney: liver ratio was 1: 5: 2. These findings show that labelled arteether was distributed quickly and localized in the cytoplasmic cortex of the kidney and homogeneously in the liver.
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    Elevation of serum transcobalamin II in patients with scrub typhus
    (2005-01-01) Cheeraratana Cheeramakara; Wanyarat Thanomsak; Kriyaporn Songmeang; Apichart Nontprasert; Charnnarong Sanghirun; Nopachai Suthisai; Suvit Areekul; Mahidol University
    Serum transcobalamin II levels were measured in scrub typhus patients. Eighteen out of fifty-two patients admitted to Maharat Nakhon Ratchasima Hospital were diagnosed with scrub typhus infection. The serum unsaturated vitamin B12 binding protein (UBBC) and total vitamin B12 binding protein (TBBC) levels in these patients were significantly higher than in normal subjects (p<0.001). The mean serum transcobalamin II level in the typhus patients was also significantly higher than in the normal subjects (p=0.004). There was a significant correlation between serum TCII levels and typhus IgM or IgG titers (p<0.05), but not to total IgM levels. These findings indicate that patients with scrub typhus had stimulation of the recticuloendothelial system as a result of a considerable increase in transcobalamin II levels.
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    Study on serum transcobalamin II in patients with murine typhus
    (2005) Cheeraratana Cheeramakara; จีระรัตน์ จีระมะกร; Kriyaporn Songmeang; กริยาพร สองเมือง; Wanyarat Thanomsak; วัลยารัตน์ ถนอมศักดิ์; Apichart Nontpraset; อภิชาต นนท์ประเสริฐ; Nopachai Suthisai; นภชัย สุทธิสัย; Charnnarong Sanghirun; ชาญณรงค์ แสงหิรัญ; Suvit Areekul; สุวิทย์ อารีย์กูล; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Radioisotopes; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine
    We measured the serum transcobalamin II in murine typhus- infected patients (n = 16) admitted to the Hospital for Tropical Diseases in 1996-1997, compared with healthy controls (n = 60). The results showed that the transcobalamin II (TCII) and total serum unsaturated vitaminB12 binding capacity (UBBC) in patients with murine typhus (2,126.5 pg/ml, range 1,262-4,568 and 3,771.5 pg/ml, range 1,576-6,763 pg/ml) were statistically significantly higher than normal subjects (987.5 pg/ml, range 678-2,000 pg/ml and 1,402 pg/ml, range 932-2,470 ml) (p<0.001). Serum TCII levels in patients (63%) were elevated during the febrile period and returned to normal post-treatment. These findings suggest that patients with murine typhus had stimulation of reticulo-endothelial system, spleen, mesenteric lymph nodes, liver and skin and then released TCII into the blood circulation. The elevation in TCII may be used for confirming a diagnosis of murine typhus.
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    Study on serum transcobalamin II in patients with murine typhus.
    (2006-12-01) Cheeraratana Cheeramakara; Kriyaporn Songmeang; Wanyarat Nakosiri; Nopachai Suthisai; Apichart Nontprasert; Suvit Areekul; Mahidol University
    We measured the serum transcobalamin II in murine typhus- infected patients (n = 16) admitted to the Hospital for Tropical Diseases in 1996-1997, compared with healthy controls (n = 60). The results showed that the transcobalamin II (TCII) and total serum unsaturated vitaminB12 binding capacity (UBBC) in patients with murine typhus (2,126.5 pg/ml, range 1,262-4,568 and 3,771.5 pg/ml, range 1,576-6,763 pg/ml) were statistically significantly higher than normal subjects (987.5 pg/ml, range 678-2,000 pg/ml and 1,402 pg/ml, range 932-2,470 ml) (p<0.001). Serum TCII levels in patients (63%) were elevated during the febrile period and returned to normal post-treatment. These findings suggest that patients with murine typhus had stimulation of reticulo-endothelial system, spleen, mesenteric lymph nodes, liver and skin and then released TCII into the blood circulation. The elevation in TCII may be used for confirming a diagnosis of murine typhus.