Publication: The evaluation of loop-mediated isothermal amplification-quartz crystal microbalance (LAMP-QCM) biosensor as a real-time measurement of HPV16 DNA
Issued Date
2016-03-01
Resource Type
ISSN
18790984
01660934
01660934
Other identifier(s)
2-s2.0-84960887737
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.229, (2016), 8-11
Suggested Citation
Patcharee Jearanaikoon, Preeda Prakrankamanant, Chanvit Leelayuwat, Surasak Wanram, Temduang Limpaiboon, Chamras Promptmas The evaluation of loop-mediated isothermal amplification-quartz crystal microbalance (LAMP-QCM) biosensor as a real-time measurement of HPV16 DNA. Journal of Virological Methods. Vol.229, (2016), 8-11. doi:10.1016/j.jviromet.2015.12.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40824
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Title
The evaluation of loop-mediated isothermal amplification-quartz crystal microbalance (LAMP-QCM) biosensor as a real-time measurement of HPV16 DNA
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Abstract
© 2015 Elsevier B.V. We have previously developed quartz crystal microbalance biosensor integrated with loop-mediated isothermal amplification (LAMP-QCM) for human papillomavirus (HPV) type58 DNA detection. Infection with HPV, particularly HPV16, remains a serious health problem due to its major risk factor contributing to cervical cancer. In the present study, LAMP-QCM biosensor was evaluated in terms of a quantitative assay for copy number of HPV16 DNA in cervical samples compared to quantitative PCR using TaqMan assay (TaqMan-qPCR). The detection limit of LAMP-QCM was found to be 10 fold more sensitive than TaqMan-qPCR with 100% specificity and 7.6% imprecision. Different plot of HPV16 DNA copy number using Bland-Altman analysis revealed 94% correlation between LAMP-QCM and qPCR. We therefore concluded that the developed LAMP-QCM biosensor provides a possible rapid and sensitive assay for HPV16 DNA quantification in a routine laboratory.
