Further studies on the ingluence of thiamine deficiency on DMN-demethylase system in the arts
4
Issued Date
1988
Copyright Date
1988
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
x, 106 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Toxicology))--Mahidol University, 1988
Suggested Citation
Chuthaporn Akathos Further studies on the ingluence of thiamine deficiency on DMN-demethylase system in the arts. Thesis (M.Sc. (Toxicology))--Mahidol University, 1988. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/103266
Title
Further studies on the ingluence of thiamine deficiency on DMN-demethylase system in the arts
Alternative Title(s)
การศึกษาระบบเอ็นไซม์ DMN-demethylase ในตับไตและอิทธิพลของการขาดวิตามินบี
Author(s)
Advisor(s)
Abstract
The DMN-demethylase system in rat kidney microsomes consisted of at least two isozymes designated as DMN-demethylase I and DMN-demethylase II which operated at low concethration (4-50 mM) and high concentration of DMN (above 50 mM) respectively. The optimum substrate concentrations determined were 4 mM for DMN-demethylase I and 200 mM for DMN-demethylase II. The activity of DMN-demethylase I and DMN-demethylase II of kidney microsomes were only 11% and 16% of the liver enzymes, respectively. In the kidney enzyme system, phenobarbital pretreatment had no effect on DMN-demethylase I and DMN-demethylase II activities. While 3-MC pretreatment incresed both DMN-demethlase I (37%) and DMN-demethylase II (30%). Thiamine deficiency enhanced both the kidney DMN-demethylase I and DMN-demethylase II activities. This effect is different from that of the liver system in which thiamine deficiency markedly enhanced the activity of DMN-demethylase II without a significant effect on DMN-demethylase I. Phenobarbital pretreatment in thiamine deficient rats produced no effects on kidney DMN-demethylase activity as also observed in the controls. For 3-MC pretreatment, only DMN-demethylase II was 22% decreased. Thiamine hydrochloride supplementation could reverse the enhanced effect of thiamine deficiency and this effect was not mediated through the level of TPP in the microsomal enzyme system.
Description
Toxicology (Mahidol University 1988)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Toxicology
Degree Grantor(s)
Mahidol University