Hierarchical Graphene/Au/Polyaniline Nanostructured Electrode for Dual-Modality Electrochemical LAMP Biosensing of Helicobacter pylori
Issued Date
2025-11-18
Resource Type
ISSN
00032700
eISSN
15206882
Scopus ID
2-s2.0-105022082545
Journal Title
Analytical Chemistry
Volume
97
Issue
45
Start Page
25148
End Page
25157
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytical Chemistry Vol.97 No.45 (2025) , 25148-25157
Suggested Citation
Kumar R.R., Wang C.Y., Bharti A.M., Hu W.C., Zhang Y.C., Yu L.S., Tungtrakarnkul P., Wu K.C.W., Chuang C.H. Hierarchical Graphene/Au/Polyaniline Nanostructured Electrode for Dual-Modality Electrochemical LAMP Biosensing of Helicobacter pylori. Analytical Chemistry Vol.97 No.45 (2025) , 25148-25157. 25157. doi:10.1021/acs.analchem.5c04200 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113220
Title
Hierarchical Graphene/Au/Polyaniline Nanostructured Electrode for Dual-Modality Electrochemical LAMP Biosensing of Helicobacter pylori
Corresponding Author(s)
Other Contributor(s)
Abstract
Helicobacter pylori (HP) infection is directly associated with over 90% of all gastric cancer (GC) cases. Currently available HP tests are prone to false negatives and are inapt for decentralization. Reliable and user-friendly detection platforms for timely diagnosis and routine monitoring are critical to patient survival. Herein, we targeted this unmet need by developing a novel biosensing platform that strategically combined the robust versatility of electrochemical techniques with the sensitivity and specificity of loop-mediated isothermal amplification (LAMP) to enable the accurate detection of HP at the point-of-care (POC). The biosensor design consisted of an underlying screen-printed carbon electrode (SCPE), sequentially modified with (i) highly conductive acid-functionalized sp2 graphene (Gr) and (ii) needle-like gold microstructures encapsulated with (iii) pH-sensitive polyaniline (PANI), assembled between laser-cut cover layers with a built-in reaction chamber for facile sample handling and detection. LAMP was performed using synthesized primers targeting the HP glmM gene. The amplification inherently generates H<sup>+</sup>, triggering pH variations, which are precisely tracked by the developed biosensor to simultaneously monitor amplicon growth and quantify HP DNA concentrations via two modes: continuous mode and segmented mode. High sensitivity in a wide linear range (1 to 107 copies/μL) with a limit of detection (LOD) of 1 copy/μL is reported. Furthermore, the excellent correlation with clinical results underscored the practical feasibility of this platform to allow reliable early diagnosis of HP infections and serve as a viable alternative to gastric endoscopy.