Publication: Subcellular localizations and time-course expression of dengue envelope and non-structural 1 proteins in human endothelial cells
Issued Date
2011-09-01
Resource Type
ISSN
10961208
08824010
08824010
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2-s2.0-79959335103
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbial Pathogenesis. Vol.51, No.3 (2011), 225-229
Suggested Citation
Jesdaporn Poungsawai, Rattiyaporn Kanlaya, Sa nga Pattanakitsakul, Visith Thongboonkerd Subcellular localizations and time-course expression of dengue envelope and non-structural 1 proteins in human endothelial cells. Microbial Pathogenesis. Vol.51, No.3 (2011), 225-229. doi:10.1016/j.micpath.2011.04.011 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11999
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Title
Subcellular localizations and time-course expression of dengue envelope and non-structural 1 proteins in human endothelial cells
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Abstract
We performed subcellular fractionation of human endothelial cells (EA.hy926) after a challenge with dengue virus serotype-2 (DEN-2) at multiplicity of infection (MOI) of 10 for 2 h. The cells were fractionated into four fractions including cytosol, membrane/organelle, nucleus and cytoskeleton, and their purity was nicely confirmed by immunofluorescence staining of markers for individual subcellular compartments. Western blot analysis of dengue envelope (E) and non-structural 1 (NS1) proteins showed that E was present only in membrane/organelle fraction, whereas NS1 was present in membrane/organelle, nucleus, and cytoskeleton fractions. Time-course study showed that E was present immediately after the challenge, whereas NS1 was present after 12 h of the challenge. Our data may provide a new roadmap for studying mechanisms of replication of dengue virus and its interactions with host cells. © 2011 Elsevier Ltd.