Publication: A novel SR protein binding site in a cis-regulatory element of HIV-1
Issued Date
2010-11-01
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ISSN
03048608
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2-s2.0-78149411486
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Virology. Vol.155, No.11 (2010), 1789-1795
Suggested Citation
Ornpreya Suptawiwat, Chompunuch Boonarkart, Prasert Auewarakul A novel SR protein binding site in a cis-regulatory element of HIV-1. Archives of Virology. Vol.155, No.11 (2010), 1789-1795. doi:10.1007/s00705-010-0765-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/29179
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Title
A novel SR protein binding site in a cis-regulatory element of HIV-1
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Abstract
We have previously described a cis enhancing sequence (CES) in the gp41 region of the HIV-1 env gene. It could enhance HIV-1 Gag expression via both rev-dependent and CTE (constitutive transport element)-dependent pathways. We identified a functionally important and conserved region in the CES that contained a predicted binding sequence for an SR protein, ASF/SF2. We show here that ASF/SF2 bound to this sequence in an electrophoretic mobility shift assay and that the putative ASF/SF2-binding sequence was required for the enhancement of Gag expression by CES and might play a role in HIV-1 posttranscriptional regulation. © 2010 Springer-Verlag.