Publication: Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species
Submitted Date
Received Date
Accepted Date
Issued Date
2013-10-01
Copyright Date
Announcement No.
Application No.
Patent No.
Valid Date
Resource Type
Edition
Resource Version
Language
File Type
No. of Pages/File Size
ISBN
ISSN
10902392
00112240
00112240
eISSN
Scopus ID
WOS ID
Pubmed ID
arXiv ID
Call No.
Other identifier(s)
2-s2.0-84883555550
Journal Title
Volume
Issue
item.page.oaire.edition
Start Page
End Page
Access Rights
Access Status
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Physical Location
Bibliographic Citation
Cryobiology. Vol.67, No.2 (2013), 244-247
Citation
Paweena Thuwanut, Sayamon Srisuwatanasagul, Grisnarong Wongbandue, Nae Tanpradit, Ampika Thongpakdee, Daraka Tongthainan, Sukanya Manee-in, Kaywalee Chatdarong (2013). Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/30978.
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species
Alternative Title(s)
Author's Affiliation
Author's E-mail
Editor(s)
Editor's Affiliation
Corresponding Author(s)
Creator(s)
Compiler
Advisor(s)
Illustrator(s)
Applicant(s)
Inventor(s)
Issuer
Assignee
Other Contributor(s)
Series
Has Part
Abstract
This study compared the effects of slow and fast freezing of testicular tissue of wild animals collected at post-mortem on testicular structure and testicular sperm. The testes of seven animals that had died in captivity; three felids (jungle cat, lion and leopard), two cervids (rusa deer and fea's muntjac) and two bovids (Sumatran serows) were cryopreserved using slow- and fast-freezing protocols. There were greater reductions in the integrity of the sperm membrane and DNA in tissues cryopreserved using slow freezing compared to fast freezing (membrane integrity reduced by 21.5. ±. 12.4% vs. 13.0. ±. 6.9%, P= 0.11 and DNA integrity reduced by 22.7. ±. 16.3% vs. 6.6. ±. 6.3%, P= 0.13). Histologically, there were similar degrees of detachment and shrinkage of the seminiferous tubules whereas, TUNEL assay revealed a tendency towards more apoptotic changes in the intra-tubular cells of tissues frozen using fast freezing compared to slow freezing (P= 0.09). In conclusion, fast freezing tended to cause less damage to testicular sperm but its protective effect on intra-tubular cells was likely compromised. This is the first report of gamete recovery in the wild and of the comparison in various wildlife species, between testicular tissues cryopreserved using different protocols. © 2013 Elsevier Inc.