Publication: A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
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Issued Date
2020-03-13
Resource Type
ISSN
14760711
Other identifier(s)
2-s2.0-85081976448
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Mahidol University
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SCOPUS
Bibliographic Citation
Annals of Clinical Microbiology and Antimicrobials. Vol.19, No.1 (2020)
Suggested Citation
Dhammika Leshan Wannigama, Cameron Hurst, Parichart Hongsing, Lachlan Pearson, Thammakorn Saethang, Naphat Chantaravisoot, Uthaibhorn Singkham-In, Sirirat Luk-In, Robin James Storer, Tanittha Chatsuwan A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa. Annals of Clinical Microbiology and Antimicrobials. Vol.19, No.1 (2020). doi:10.1186/s12941-020-00350-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/54619
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Title
A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
Abstract
© 2020 The Author(s). Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.