cDNA expression library and immunoscreening of thai paragonimus heterotremus
4
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Kanokkarn Pothong, กนกกาญจน์ โพธิ์ทอง, Paron Dekumyoy, พารณ ดีคำย้อย, Thareerat Kalambaheti, ธารีรัตน์ กะลัมพะเหติ, Dorn Watthanakulpanich, ดร วัฒนกุลพานิชย์, Chalit Komalmisra, ชลิต โกมลมิศร์, Timothy P Yoshino (2014). cDNA expression library and immunoscreening of thai paragonimus heterotremus. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63173
Title
cDNA expression library and immunoscreening of thai paragonimus heterotremus
Abstract
A cDNA expression library of Paragonimus heterotremus, the main causative
agent of human paragonimiasis in Thailand, was performed to produce
recombinant antigens. To identify the antigenic clones, immunized sera
were tested using immunoscreening. Ten positive clones were selected, and the
inserted genes partially sequenced. The sequencing data were analyzed using BLASTp
searches. Six clones were found to contain a gene insert that encoded a protein
similar to that found in helminthic worms (Clonorchis sinensis, Schistosoma mansoni,
Schistosoma japonicum, Echinococcus multilocularis, Brugia malayi), two clones
similar to Toxoplasma gondii, while two more clones were shown to encode proteins
similar to that of Plasmodium chabaudi chabaudiand Microcystis spp. (uncultured).
The clones will be further studied in term of protein expression through recombinant
antigen evaluation using human paragonimiasis and other parasitic infection sera.
Description
Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 278.