Determination of ivermectin in plasma and whole blood using LC-MS/MS
Issued Date
2024-01-01
Resource Type
eISSN
2398502X
Scopus ID
2-s2.0-85205737374
Journal Title
Wellcome Open Research
Volume
9
Rights Holder(s)
SCOPUS
Bibliographic Citation
Wellcome Open Research Vol.9 (2024)
Suggested Citation
Kaewkhao N., Hanpithakpong W., Tarning J., Blessborn D. Determination of ivermectin in plasma and whole blood using LC-MS/MS. Wellcome Open Research Vol.9 (2024). doi:10.12688/wellcomeopenres.20613.2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101612
Title
Determination of ivermectin in plasma and whole blood using LC-MS/MS
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Abstract
Background: Ivermectin is a widely used drug for the treatment of helminthiasis and filariasis worldwide, and it has also shown promise for malaria elimination through its potent mosquito-lethal activity. The objective of this study was to develop and validate a high-throughput and sensitive method to quantify ivermectin in plasma and whole blood samples, using automated sample extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: Phospholipids were removed in patient whole blood (100 µl) and plasma (100 µl) samples using a 96-well plate Hybrid-solid phase extraction technique. Ivermectin and its isotope-labelled internal standard (ivermectin-D2) were separated on an Agilent Poroshell 120 EC-C18 50mm × 3.0mm I.D. 2.7µm, using a mobile phase of acetonitrile: ammonium formate 2 mM containing 0.5% formic acid (90: 10, v/v). Detection was performed using a triple quadrupole mass spectrometer in the positive ionization mode. Results: The method was validated in the concentration range 0.970 - 384 ng/ml in both plasma and whole blood matrices. Intra- and inter-batch precisions during the validation were below 15%. There was no carryover or matrix effects detected. Ivermectin is a stable compound and results showed no degradation in the different stability tests. Conclusions: The validated method proved to have high sensitivity and precision, good selectivity and to be suitable for clinical application or laboratory quantification of ivermectin in plasma or whole blood samples.