Publication: Development of new, broadly reactive, rapid IgG and IgM lateral flow assays for diagnosis of scrub typhus
Issued Date
2012-07-01
Resource Type
ISSN
00029637
Other identifier(s)
2-s2.0-84863741229
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.87, No.1 (2012), 148-152
Suggested Citation
Saowaluk Silpasakorn, Nujorn Srisamut, Pattama Ekpo, Zhiwen Zhang, Chien Chung Chao, Wei Mei Ching, Yupin Suputtamongkol Development of new, broadly reactive, rapid IgG and IgM lateral flow assays for diagnosis of scrub typhus. American Journal of Tropical Medicine and Hygiene. Vol.87, No.1 (2012), 148-152. doi:10.4269/ajtmh.2012.11-0583 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14298
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Title
Development of new, broadly reactive, rapid IgG and IgM lateral flow assays for diagnosis of scrub typhus
Abstract
We evaluated the diagnostic accuracy of two broadly reactive rapid immunochromatographic tests (ICTs) for detection of IgM and IgG against Orientia tsutsugamushi by using archived acute-phase serum samples from 102 patients with laboratory-confirmed scrub typhus, and from 62 archived serum samples from patients with other causes of fever as a negative control. These ICTs were constructed by using a mixture of recombinant proteins: 1) C1, a chimeric protein containing epitopes of the 56-kD antigen from Karp and TA763 strains; 2) Ktr56; and 3) Gmr56. Sensitivities of the ICTs for detection of IgM and IgG were 90.2% (95% confidence interval [CI] = 84.4-96.0%) and 86.3% (95% CI = 80.9-93.8%), respectively. Specificities were 85.5% (95% CI = 73.9-92.2%) and 96.8% (95% CI = 90.3-100%), respectively. Both assays were more sensitive and specific than the standard immune immunofluorescence assay for the early diagnosis of scrub typhus. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.