Publication:
A method to differentiate between anti-C1q antibodies and C1q-binding immune complexes using collagenase-digested solid phase C1q

Issued Date

1991-04-25

Resource Type

Article

ISSN

00221759

DOI

10.1016/0022-1759(91)90164-B

Other identifier(s)

2-s2.0-0025780219

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Immunological Methods. Vol.138, No.2 (1991), 165-171

Suggested Citation

Johannes E. Menzel, Othmar Scherak, Gernot Kolarz, Fritz Gamerith, Uriwan Youngchaiyud A method to differentiate between anti-C1q antibodies and C1q-binding immune complexes using collagenase-digested solid phase C1q. Journal of Immunological Methods. Vol.138, No.2 (1991), 165-171. doi:10.1016/0022-1759(91)90164-B Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21989

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Title

A method to differentiate between anti-C1q antibodies and C1q-binding immune complexes using collagenase-digested solid phase C1q

Author(s)

Johannes E. Menzel
 Othmar Scherak
 Gernot Kolarz
 Fritz Gamerith
 Uriwan Youngchaiyud

Other Contributor(s)

Medizinische Universitat Wien, Instituts fur Immunologie
 Ludwig Boltzmann Institute
 Mahidol University

Abstract

A method for the detection of circulating immune complexes in the presence of autoantibodies to C1q is described. Solid phase C1q-digestion with bacterial collagenase results in the elimination of the collagen-like region of C1q. Binding of model immune complexes to this modified solid phase C1q is practically unaltered, while reactivity of anti-C1q antibodies is abolished by this procedure. In conjunction with an ELISA using the collagen-like region of C1q as antigen this modified C1q solid phase assay may be used to determine immune complexes and anti-C1q antibodies in the sera of patients with autoimmune rheumatic diseases. © 1991.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/21989

Collections

Scopus 1991-2000