Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.6 (2009), 1254-1258
Suggested Citation
Pannamas Maneekan, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, Jaranit Kaewkangwal, Natthanej Luplertlop Development of single-tube mutiplex RT-PCR for dengue virus typing. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.6 (2009), 1254-1258. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27864
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Development of single-tube mutiplex RT-PCR for dengue virus typing
This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.
