Publication: Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
Issued Date
2020-10-01
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ISSN
18767753
18735061
18735061
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2-s2.0-85089190010
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Research. Vol.48, (2020)
Suggested Citation
Chanchao Lorthongpanich, Chuti Laowtammathron, Nittaya Jiamvoraphong, Pimonwan Srisook, Pimjai Chingsuwanrote, Phatchanat Klaihmon, Supaporn Waeteekul, Yaowalak U-pratya, Surapol Issaragrisil Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3. Stem Cell Research. Vol.48, (2020). doi:10.1016/j.scr.2020.101950 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57672
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Title
Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
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Abstract
© 2020 The Authors In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we established a stable LATS1 knockdown (LATS1-KD) iPSC from the MUSIi012-A cell line. The LATS1-KD iPSC MUSIi012-A-3 that was developed maintained both the normal karyotype and the pluripotent phenotype, and retained the ability to differentiate into all three embryonic germ layers.