Publication: Investigating lysozyme amyloid fibrillization by electrochemical impedance spectroscopy for application in lysozyme sensor
Issued Date
2021-11-15
Resource Type
ISSN
15726657
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2-s2.0-85117614092
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Electroanalytical Chemistry. Vol.901, (2021)
Suggested Citation
Ibrar Alam, Benchaporn Lertanantawong, Worapot Prongmanee, Tossaporn Lertvanithphol, Mati Horprathum, Thana Sutthibutpong, Piyapong Asanithi Investigating lysozyme amyloid fibrillization by electrochemical impedance spectroscopy for application in lysozyme sensor. Journal of Electroanalytical Chemistry. Vol.901, (2021). doi:10.1016/j.jelechem.2021.115799 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76496
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Title
Investigating lysozyme amyloid fibrillization by electrochemical impedance spectroscopy for application in lysozyme sensor
Abstract
Amyloid fibrils have garnered increasing attention as viable building blocks for designing and synthesizing of biomedical material. The structure of amyloid fibril of most of the proteins are well known, but the electrical property of their conformational changes during synthesis is not fully understood. Herein, we investigated this relationship by using non-Faradaic electrochemical impedance spectroscopy (EIS). Lysozyme (Lyz) from hen egg white was used as a model protein and incubated under heat (60 °C) at pH 2 which led to the amyloid fibrillization. Morphological changes during fibrillization were confirmed by AFM. Raman spectroscopy was used to distinguish amyloid fibrils from their native and amorphous structures. For EIS measurement, graphene oxide coated screen-printed electrode (GO/SPE) was immobilized with Lyz aggregates obtained from different incubation times. The charge transfer resistance (Rct) of incubated Lyz was measured to determine the fibrillization pathway of amyloid fibril i.e., unfolding and nucleation, protofibril (PF, a short fibril) and mature fibril. Next, PF-modified GO/SPE (PF/GO/SPE) was demonstrated to determine Lyz molecules dissolved in PBS and in wine using EIS. The dependence of Rct of PF/GO/SPE on Lyz concentration was reported. Linear range and limit of detection (LOD) of detecting Lyz molecules in PBS and in wine were the same. The linear range and LOD were 0.005–0.08 μM and 0.001 μM, respectively. PF/GO/SPE sensor was found to have good selectivity to recognize Lyz. These findings show that PF/GO/SPE sensor provides an attractive component for future electrochemical sensing devices.