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Publication Metadata only Enzyme-linked immunosorbent assay for detection of antibody to Gnathostoma antigen in patients with intermittent cutaneous migratory swelling(1986-08-06) A. Dharmkrong-At; S. Migasena; P. Suntharasamai; D. Bunnag; R. Priwan; S. Sirisinha; Mahidol UniversityA sensitive enzyme-linked immunosorbent assay is described for the detection of immunoglobulin G antibody to Gnathostoma antigen in the sera of patients with intermittent cutaneous migratory swelling who were suspected to being infected... by the tissue nematode Gnathostoma spinigerum. The antigen used was a crude somatic aqueous extract of the third larval stage obtained from naturally infected eels. The 1:320 dilution of all sera from 46 patients gave enzyme-linked immunosorbent assay valuesPublication Metadata only Evaluation of a monoclonal antibody-based enzyme-linked immunosorbent assay for the diagnosis of Opisthorchis viverrini infection in an endemic area(1995-01-01) S. Sirisinha; R. Chawengkirttikul; M. R. Haswell-Elkins; D. B. Elkins; S. Kaewkes; P. Sithithaworn; Mahidol UniversityA monoclonal antibody based enzyme-linked immunosorbent assay (MAb-ELISA) was evaluated for its potential in the diagnosis of opisthorchiasis in an area endemic for Opisthorchis viverrini infection. The method, based on the detection of the 89-kD OPublication Metadata only Rapid antigen detection assay for identification of Burkholderia (Pseudomonas) pseudomanas infection(1996-12-01) A. Anuntagool; P. Intachote; P. Naigowit; S. Sirisinha; Chulabhorn Research Institute; Mahidol University; Thailand Ministry of Public HealthA simple antigen detection test was developed for rapid diagnosis of melioidosis caused by Burkholderia (Pseudomonas) pseudomallei infection. The method was based on the use of a specific monoclonal antibody in a sandwich enzyme-linked immunosorbent... assay to capture antigen in clinical specimens culture positive for B. pseudomallei. The results showed the sensitivity and specificity of the test to be 75 and 98%, respectively.Publication Metadata only Immunological analysis of Opisthorchis and Clonorchis antigens(1990-01-01) S. Sirisinha; D. Sahassananda; Faculty of Scie; Mahidol University; Korea UniversityImmunoreactive components of Opisthorchis viverrini and Clonorchis sinensis were analysed by enzyme-linked immunosorbent assay (ELISA), radioimmunoprecipitation and immunoblotting. Somatic extracts from these two liver flukes as well as from otherPublication Metadata only Analysis of Opisthorchis viverrini antigens: physicochemical characterization and antigen localization.(1987-12-01) S. Wongratanacheewin; S. Sirisinha; Mahidol UniversityAntigens of Opisthorchis viverrini were identified and characterized by enzyme-linked immunosorbent assay, polyacrylamide gel electrophoresis following radioimmuno-precipitation, and indirect immunofluorescence. The immunoreactive protein with aPublication Metadata only Immunodiagnosis of opisthorchiasis.(1991-12-01) S. Sirisinha; R. Chawengkirttikul; R. Sermswan; Mahidol UniversityMonoclonal antibody-based enzyme-linked immunosorbent assay and DNA dot blot hybridization techniques were developed and evaluated for their potential in the detection of Opisthorchis viverrini. A mixture of IgG monoclonal antibodies specificPublication Metadata only Gel-filtration chromatography of Salmonella protein antigens and their implication in immunodiagnosis.(1987-12-01) S. Kanjanahareutai; S. Sarasombath; S. Sirisinha; Mahidol Universityand, together with the first peak, subjected to polyacrylamide gel electrophoresis and immunoprecipitation with both homologous and heterologous rabbit antisera. The extent of immunological cross reactivities was determined by enzyme-linked immunosorbent assayPublication Metadata only Production of monoclonal antibody specific to Campylobacter jejuni and its potential in diagnosis of Campylobacter enteritis(1995-01-01) S. C. Chaiyaroj; T. Sirisereewan; N. Jiamwatanasuk; S. Sirisinha; Mahidol University. By immunoprecipitation, MAb 3G6 identified a major protein band of molecular weight 45 kDa and also gave a slight reactivity with 30 and 55 kDa proteins. Using an indirect enzyme linked immunosorbent assay, MAb 3G6 was able to detect C. jejuni suspended in stool withoutPublication Metadata only IgE responses in human gnathostomiasis(1987-01-01) M. H N E Soesatyo; W. Rattanasiriwilai; P. Suntharasamai; S. Sirisinha; Mahidol University; Chulalongkorn UniversitySpecific IgE antibody levels in the serum of patients with proven gnathostomiasis and in those with intermittent cutaneous migratory swellings were determined by the enzyme-linked immunosorbent assay (ELISA) using aqueous extracts of GnathostomaPublication Metadata only Detection of Opisthorchis viverrini by monoclonal antibody-based ELISA and DNA hybridization(1991-01-01) S. Sirisinha; R. Chawengkirttikul; R. Sermswan; S. Amornpant; S. Mongkolsuk; S. Panyim; Mahidol UniversityMonoclonal antibody-based enzyme-linked immunosorbent assay and DNA hybridization techniques were developed and evaluated for their potential in the detection of Opisthorchis viverrini infection in humans. A mixture of three IgG1monoclonal