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Publication Metadata only Expression of Aquaporin-1, -2 and -4 in Mice with a Spontaneous Mutation Leading to Hydronephrosis(2012) S. Ampawong; A. Klincomhum; W. Likitsuntonwong; O. Singha; T. Ketjareon; Y. Panavechkijkul; K.-M. Zaw; K. Kengkoom; Mahidol University. National Laboratory Animal Center. Veterinary Medical Care Office; Mahidol University. National Laboratory Animal Center. Quality Assurance Office; Mahidol University. National Laboratory Animal Center. Academic Service OfficeThis study investigates the expression of aquaporin-1, -2 and -4 in mice with a spontaneously-arising mutation that leads to hydronephrosis (ICR/Mlac-hydro mice). The mutant mice developed bilateral non-obstructive hydronephrosis without evidencePublication Metadata only Multiple Mutations in the rpoB gene of Mycobacterium Leprae strains from leprosy patients in Thailand(2000-09-01) Pongrama Ramasoota; Waranya Wongwit; Prasert Sampunachot; Komes Unnarat; Maeya Ngamying; Stefan B. Svenson; Mahidol University; Prapradaeng Hospital; Leprosy Division; Swedish University of Agricultural Sciences Faculty of Veterinary Medicine and Animal Science; Sveriges lantbruksuniversitetA new finding is reported of multiple mutations in the rpoB gene of 9 Mycobacterium leprae strains from leprosy patients in Thailand, who did not respond to therapy even when rifampicin, the main drug in multi-drug therapy was used. By means... of sequence analysis of 9 Thai M. leprae strains, various mutations in 289 bps of the rpoB gene revealed forms of mutation never before described, such as multiple mutations (ie, mutation at two, three, six, seven, eight and nine positions in the rpoB genePublication Metadata only Insertion of common mutations into the human β-globin locus using GET Recombination and an EcoRI endonuclease counterselection cassette(2003-02-27) Duangporn Jamsai; Mikhail Nefedov; Kumaran Narayanan; Michael Orford; Suthat Fucharoen; Robert Williamson; Panos A. Ioannou; Royal Children's Hospital, Melbourne; Mahidol University; Universiti Malaysia Sarawak; Cyprus Institute of Neurology and GeneticsA large number of mutations have been described in the human β-globin locus causing thalassemia or various hemoglobinopathies. However, only a very limited number of these mutations have been studied in animal model systems in the context... of the human β-globin locus. We report here the use of the GET Recombination system with an EcoRI/KanRcounterselection cassette to facilitate the introduction of the HbE (codon 26, GAG→AAG mutation and the codon 41-42 (-TTCT) deletion, two mutations foundPublication Metadata only A humanized mouse model for a common β0-thalassemia mutation(2005-01-01) Duangporn Jamsai; Faten Zaibak; Wantana Khongnium; Jim Vadolas; Lucille Voullaire; Kerry J. Fowler; Sophie Gazeas; Suthat Fucharoen; Robert Williamson; Panayiotis A. Ioannou; University of Melbourne; Mahidol University; Cyprus Institute of Neurology and GeneticsAccurate animal models that recapitulate the phenotype and genotype of patients with β-thalassemia would enable the development of a range of possible therapeutic approaches. Here we report the generation of a mouse model carrying the codons 41-42... (-TTCT) β-thalassemia mutation in the intact human β-globin locus. This mutation accounts for approximately 40% of β-thalassemia mutations in southern China and Thailand. We demonstrate a low level of production of γ-globins from the mutant locus in dayPublication Metadata only Expression of foot and mouth disease virus nonstructural polyprotein 3ABC with inactive 3C pro in Escherichia coli(2011-11-01) Ladawan Sariya; Nattarat Thangthumniyom; Worawidh Wajjwalku; Wilairat Chumsing; Pongrama Ramasoota; Porntippa Lekcharoensuk; Mahidol University; Kasetsart UniversityNonstructural 3ABC protein of foot and mouth disease virus (FMDV) was widely used to differentiate vaccinated from natural FMDV-infected animals. 3ABC is a polyprotein which is auto-processed to 3A, three copies of 3B and 3C pro by 3C pro protease.... The 3ABC gene was cloned and expressed in Escherichia coli as native or mutated 3ABC (mu3ABC) forms. Cysteine residues 142 and 163 of the catalytic triad within the 3C pro of mu3ABC were changed to serine and glycine, respectively, to inhibit itsPublication Metadata only Comparative Genomic Analysis and a Novel Set of Missense Mutation of the Leptospira weilii Serogroup Mini From the Urine of Asymptomatic Dogs in Thailand(2021-10-18) Alongkorn Kurilung; Vincent Perreten; Nuvee Prapasarakul; Siriraj Hospital; Chulalongkorn University; University of BernLeptospira weilii belongs to the pathogenic Leptospira group and is a causal agent of human and animal leptospirosis in many world regions. L. weilii can produce varied clinical presentations from asymptomatic through acute to chronic infections... into the missense mutations, focusing on the known pathogenesis-related genes. Whole genome sequences revealed that the CUDO6 and CUD13 strains each contained two chromosomes and one plasmid, with average genome size and G+C content of 4.37 Mbp and 40Publication Metadata only Molecular epidemiology of gibbon hepatitis B virus transmision(2003-01-01) Suwanna Noppornpanth; Bart L. Haagmans; Parvapan Bhattarakosol; Parntep Ratanakorn; Hubert G.M. Niesters; Albert D.M.E. Osterhaus; Yong Poovorawan; Chulalongkorn University; Erasmus University Rotterdam; Mahidol University. Some of the chronic animals were found to be anti-HBc (HBV core antigen) negative (4 of 19), while precore promoter point mutations (nt 1762 or 1764) were determined in four animals by RFLP analysis. Phylogenetic tree analysis of the complete surface... analysed in 101 captive gibbons in Thailand. Approximately 40% of these animals showed at least one marker of HBV infection; 19 animals were chronic HBV carriers, characterized by elevated levels of alanine amino transferase and the presence of HBV DNAPublication Metadata only Personalised genetic intervention for duchenne muscular dystrophy: Antisense oligomers and exon skipping(2009-12-01) Chalermchai Mitrpant; Sue Fletcher; Steve D. Wilton; University of Western Australia; Edith Cowan University, Joondalup; Mahidol Universitycorrupted by a nonsense mutation or microinsertion/deletion. This review discusses the evolution of oligomer induced exon skipping, including in vitro applications, evaluation of different oligomer chemistries, the treatment of animal models and alternative...Duchenne muscular dystrophy (DMD) arises from protein-truncating mutations in the large dystrophin gene that preclude synthesis of a functional protein that primarily stabilizes muscle fibre membranes. The absence of dystrophin leads to this mostPublication Metadata only Functional analysis of XRCC4 mutations in reported microcephaly and growth defect patients in terms of radiosensitivity(2021-05-01) Anie Day D.C. Asa; Rujira Wanotayan; Mukesh Kumar Sharma; Kaima Tsukada; Mikio Shimada; Yoshihisa Matsumoto; Tokyo Institute of Technology; Mahidol University; S.P.C. Govt. Collegeto be associated with immunodeficiency in human as well as in model animals. Several human patients with mutations in XRCC4 were reported to exhibit microcephaly and growth defects, but unexpectedly showed normal immune function. Here, to evaluate the functionality...Non-homologous end joining is one of the main pathways for DNA double-strand break (DSB) repair and is also implicated in V(D)J recombination in immune system. Therefore, mutations in non-homologous end-joining (NHEJ) proteins were foundPublication Metadata only Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR(2013-01-01) Dettachai Ketpun; Achariya Sailasuta; Prapruddee Piyaviriyakul; Nattawat Onlamoon; Kovit Pattanapanyasat; Chulalongkorn University; Mahidol University© 2013 Dettachai Ketpun et al. A 13-year-old, poodle-mixed, male dog was referred to the oncology unit in our faculty's small animal teaching hospital with the problem of rapid recurrent MCT. The owner and the veterinarian would like to use a... tyrosine kinase inhibitor (TKI) for the dog. Therefore, fine-needle aspiration (FNA) was performed to collect the MCT cells and these cells were submitted to our laboratory for the detection of internal-tandem-duplicated (ITD) mutation of exon-11 in c-kit