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Publication Metadata only Different states of sarcoplasmic reticulum membrane in the presence of acetyl phosphate and adenosine triphosphate(1977-09-01) Yongyuth Yuthavong; Pailin Ucchin; Rossukon Sari; Mahidol UniversityIn the presence of acetyl phosphate, approximately 0.8 extra sulphydryl groups/10 5 g protein of sarcoplasmic reticulum membrane vesicles are exposed to reaction with N-ethylmaleimide, whereas in the presence of ATP approximately 0.6 groups/10 5 g... protein are protected. Dithiobis (nitrobenzoic acid) reacts with the membrane sulphydryl groups more slowly in the presence of ATP than in the presence of acetyl phosphate or in the absence of substrate. Sarcoplasmic reticulum membrane is degradedPublication Metadata only Protein and lipid composition of sarcoplasmic reticulum from dystrophic muscles of vitamin E-deficient rabbits(1980-01-01) P. Liewsaree; Y. Yuthavong; P. Wilairat; P. Komaratat; Mahidol UniversityThe protein and lipid compositions of sarcoplasmic reticulum (SR) membranes from vitamin E-deficient and control rabbits were analyzed. In vitamin E-deficient rabbits, sodium dodecyl-sulfate polyacrylamide gel-electrophoresis and sulfhydryl contentPublication Metadata only Regulatory role of ovarian sex hormones in calcium uptake activity of cardiac sarcoplasmic reticulum(2006-09-13) Tepmanas Bupha-Intr; Jonggonnee Wattanapermpool; Mahidol Universityintracellular Ca2+mobilization, the sarcoplasmic reticulum (SR) Ca2+uptake and SR Ca2+-ATPase (SERCA) activity were determined in 10-wk ovariectomized rat hearts. With the use of left ventricular homogenate preparations, a significant suppression of maximum SRPublication Metadata only Comparison of sarcoplasmic reticulum Ca2+-adenosine triphos-phatase from vitamin E-deficient dystrophic rabbit skeletal muscle with iron-ascorbate-treated and untreated enzyme(1988-01-01) Warawadee Nirdnoy; Prayad Komaratat; Prapon Wilairat; Mahidol UniversitySarcoplasmic reticulum Ca 2+ ATPase from rabbit skeletal muscle has an Arrhenius curve of enzyme activity with a discontinuity at about 20°C. Preparations treated with FeSO 4 and ascorbic acid and from a vitamin E-deficient dystrophic rabbit have 22Publication Metadata only Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation(2017-10-01) Sukanya Phungphong; Anusak Kijtawornrat; Pieter P. de Tombe; Jonggonnee Wattanapermpool; Tepmanas Bupha-Intr; Sunit Suksamrarn; Mahidol University; Chulalongkorn University; Loyola University Stritch School of Medicine; Srinakharinwirot Universitysarcoplasmic reticulum (SR) membrane preparation, α-mangostin inhibited SR Ca2+-ATPase activity in a dose-dependent manner (IC50of 6.47 ± 0.7 μM). Its suppressive effect was specific to SR Ca2+-ATPase but not to myofibrillar Ca2+-ATPase. Using isolatedPublication Metadata only Altered property of sarcoplasmic Ca-ATPase from vitamin E-deficient dystrophic rabbit is associated with the protein and not the lipid component(1983-01-01) K. Patipaporn; P. Wilairat; P. Komaratat; Mahidol UniversityThe effects of temperature on reconstituted sarcoplasmic Ca-ATPase preparations from vitamin E-deficient dystrophic and control rabbits were studied. Delipidated Ca-ATPase from vitamin E-deficient sarcoplasmic reticulum (SR) reconstituted with lipid... with lipid of vitamin E-deficient SR showed a reduction in activity by retained the discontinuity in the Arrhenius plot. These results indicated that the altered property of sarcoplasmic Ca-ATPase from vitamin E-deficient dystrophic rabbit was associatedPublication Metadata only Effects of vitamin E deficiency on the activities of lipid-requiring enzymes in rabbit liver and muscle(1979-12-01) C. Vajanamarhutue; P. Wilairat; P. Komaratat; Mahidol UniversityThe effects of vitamin E deficiency on membrane integrity were studied by examining the temperature dependence of membrane-bound enzyme activities in liver mitochondria and microsome and in muscle sarcoplasmic reticulum. In vitamin E-deficient... C reductase (EC 1.6.99.3) and sarcoplasmic reticulum Ca-ATPase were reduced in comparison to control rabbits. Arrhenius plots of activity against temperature yielded a linear plot over the range 10 to 40°C in the case of β-hydroxybutyratePublication Metadata only Testosterone regulates cardiac contractile activation by modulating SERCA but not NCX activity(2013-02-01) Namthip Witayavanitkul; Warunya Woranush; Tepmanas Bupha-Intr; Jonggonnee Wattanapermpool; Mahidol Universitytension was also suppressed in ORX rats without changes in myofilament Ca2+sensitivity and passive stiffness of the heart. Using a sarcoplasmic reticulum-enriched membrane preparation, the maximum thapsigargin-sensitive SERCA activity of the ORX rat was 27... relaxation, Ca2+-handling activities by sarco-(endo)plasmic reticulum Ca2+-ATPase (SERCA) and the Na+/Ca2+exchanger (NCX) were evaluated in the ventricular muscle of 10-wk-old ORX rats with and without testosterone supplementation (2.5 mg/kg testosteronePublication Metadata only Moderate intensity of regular exercise improves cardiac SR Ca2+ uptake activity in ovariectomized rats(2009-10-01) Tepmanas Bupha-Intr; Jitanan Laosiripisan; Jonggonnee Wattanapermpool; Mahidol UniversityThe impact of regular exercise in protecting cardiac deteriorating results of female sex hormone deprivation was evaluated by measuring changes in intracellular Ca2+removal activity of sarcoplasmic reticulum (SR) in ovariectomized rats following 9Publication Metadata only Possible role of the Ca2+/Mn2+ P-type ATPase Pmr1p on artemisinin toxicity through an induction of intracellular oxidative stress(2019-03-29) Onnicha Pongwattanakewin; The Phyu; Suchanya Suesattayapirom; Laran T. Jensen; Amornrat N. Jensen; Mahidol Universitysarcoplasmic endoplasmic reticulum Ca2+-transporting ATPase, are associated with increased tolerance to artemisinin. We utilized Saccharomyces cerevisiae as a model to examine the involvement of Pmr1p, a functional homolog of PfATP6, on the toxicity
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