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Item Metadata only Induced mutation of crude oil biodegrading bacteria (Pseudomonas aeruginosa)(Mahidol University. Mahidol University Library and Knowledge Center, 2004) Apiradee Suwanwong; Prayad PokethitiyookThe objective of this research was to find techniques to enhance oil biodegradation in petroleum contaminated soil. Induced mutation by UV irradiation of Pseudomonas aeruginosa MU 01 was performed. The UV dose that caused the mutation was 3,000 μ JPublication Metadata only Expression of Aquaporin-1, -2 and -4 in Mice with a Spontaneous Mutation Leading to Hydronephrosis(2012) S. Ampawong; A. Klincomhum; W. Likitsuntonwong; O. Singha; T. Ketjareon; Y. Panavechkijkul; K.-M. Zaw; K. Kengkoom; Mahidol University. National Laboratory Animal Center. Veterinary Medical Care Office; Mahidol University. National Laboratory Animal Center. Quality Assurance Office; Mahidol University. National Laboratory Animal Center. Academic Service OfficeThis study investigates the expression of aquaporin-1, -2 and -4 in mice with a spontaneously-arising mutation that leads to hydronephrosis (ICR/Mlac-hydro mice). The mutant mice developed bilateral non-obstructive hydronephrosis without evidencePublication Metadata only Multiple Mutations in the rpoB gene of Mycobacterium Leprae strains from leprosy patients in Thailand(2000-09-01) Pongrama Ramasoota; Waranya Wongwit; Prasert Sampunachot; Komes Unnarat; Maeya Ngamying; Stefan B. Svenson; Mahidol University; Prapradaeng Hospital; Leprosy Division; Swedish University of Agricultural Sciences Faculty of Veterinary Medicine and Animal Science; Sveriges lantbruksuniversitetA new finding is reported of multiple mutations in the rpoB gene of 9 Mycobacterium leprae strains from leprosy patients in Thailand, who did not respond to therapy even when rifampicin, the main drug in multi-drug therapy was used. By means... of sequence analysis of 9 Thai M. leprae strains, various mutations in 289 bps of the rpoB gene revealed forms of mutation never before described, such as multiple mutations (ie, mutation at two, three, six, seven, eight and nine positions in the rpoB geneItem Metadata only Modulating potential on urethane induced mutation in short term test using drosophila melanogaster or soy milk, soy yogurt and tofu(1999) Kaew Kangsadalampai; Prapasri Laohavechvanich; Chatkaew Sopa; แก้ว กังสดาลอำไพ; ประภาศรี เลาหเวชวานิช; ฉัตรแก้ว โสภาSoybean known for its protein is being recognized as having potential roles in the prevention and treatment of chronic diseases, most notably cancers and heart disease. The study on possible inhibitory effects of soymilk, soy yogurt, and tofu against mutagenicity of any genotoxins are required to support such hypothesis. Three-day old trans-heterozygous Drosophila larva obtained from the virgin ORR;flr3 females and mwh males were transferred to test tubes containing a sample and urethane (URE) for simultaneous feeding study; while in the prefed study, the virgin females and males were mated on medium containing samples to obtain three-day old larva which were brought up on regular medium containing URE. After metamorphosis, the adult flies bearing the trans-heterozygous (mwh+/+flr3) indicated with round wings were examined for the mutant wing spots. The frequencies of total spots from flies fed URE (2,200, 4,500, and 6,700 ppm) with tofu (2 g/tube), soymilk (2 ml/tube), and soy-yogurt (2g/tube) were less than that of URE treated group. Percent inhibitions of induced wing sports in prefed studies were lower than those of simultaneous administration studies. The inhibition calculated as percents reduction according to the concurrent running standard URE (2200 – 6700 ppm) in simultaneous administration studies of tofu, soymilk and soy yogurt are 64 - 77%, 67 – 79% and 43 – 71% respectively; while those of prefed studies are 8 – 73%, 46 – 66% and 31 – 48% respectively. The data indicated that tofu, soymilk, and soy-yogurt decreased the mutagenicity induced by URE. However, all samples could not reduce the mutagenicity of URE to be the value of sample control groups.Publication Metadata only Insertion of common mutations into the human β-globin locus using GET Recombination and an EcoRI endonuclease counterselection cassette(2003-02-27) Duangporn Jamsai; Mikhail Nefedov; Kumaran Narayanan; Michael Orford; Suthat Fucharoen; Robert Williamson; Panos A. Ioannou; Royal Children's Hospital, Melbourne; Mahidol University; Universiti Malaysia Sarawak; Cyprus Institute of Neurology and GeneticsA large number of mutations have been described in the human β-globin locus causing thalassemia or various hemoglobinopathies. However, only a very limited number of these mutations have been studied in animal model systems in the context... of the human β-globin locus. We report here the use of the GET Recombination system with an EcoRI/KanRcounterselection cassette to facilitate the introduction of the HbE (codon 26, GAG→AAG mutation and the codon 41-42 (-TTCT) deletion, two mutations foundItem Metadata only Sergentomyia khawi: a potential vector for Leishmania and Trypanosoma parasites affecting humans and animals and insecticide resistance status in endemic areas of Songkhla, southern Thailand(2024-12-01) Phumee A.; Sutthanont N.; Chitcharoen S.; Sawaswong V.; Boonserm R.; Ayuyoe P.; Cantos-Barreda A.; Siriyasatien P.; Phumee A.; Mahidol Universitysand flies were then identified to species level using molecular techniques. Subsequent analyses included the detection of pathogens and the identification of pyrethroid resistance mutations within the voltage-sensitive sodium channel (Vgsc) domain IIS6..., or F1020S mutations were detected in Vgsc gene. Conclusions: The results of this study provide valuable information on sand fly species and the continuous circulation of Leishmania spp. and Trypanosoma spp. in Songkhla, southern Thailand. MoreoverItem Metadata only Assessing Coagulation Parameters in Healthy Asian Elephants (Elephas maximus) from European and Thai Populations(2022-02-01) Jesus S.A.; Schmidt A.; Fickel J.; Doherr M.G.; Boonprasert K.; Thitaram C.; Sariya L.; Ratanakron P.; Hildebrandt T.B.; Mahidol Universitysuch values for diseased animals in a very short time, is of great importance. We analyzed prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen concentrations using a portable and fast point-of-care analyzer (VetScan Pro) in 127... were not expected to have biological or clinical implications. We have also sequenced the coagulation factor VII gene of 141 animals to assess the presence of a previously reported hereditary coagulation disorder in Asian elephants and to investigatePublication Metadata only A humanized mouse model for a common β0-thalassemia mutation(2005-01-01) Duangporn Jamsai; Faten Zaibak; Wantana Khongnium; Jim Vadolas; Lucille Voullaire; Kerry J. Fowler; Sophie Gazeas; Suthat Fucharoen; Robert Williamson; Panayiotis A. Ioannou; University of Melbourne; Mahidol University; Cyprus Institute of Neurology and GeneticsAccurate animal models that recapitulate the phenotype and genotype of patients with β-thalassemia would enable the development of a range of possible therapeutic approaches. Here we report the generation of a mouse model carrying the codons 41-42... (-TTCT) β-thalassemia mutation in the intact human β-globin locus. This mutation accounts for approximately 40% of β-thalassemia mutations in southern China and Thailand. We demonstrate a low level of production of γ-globins from the mutant locus in dayItem Metadata only Mutagenic tests of stevioside and steviol(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Jittima Pimbua; Thirayudh GlinsukonThe mutagenicity of stevioside, a constituent of Stevia rebauaians, and steviol, the aglycone of stevio-sice, was determined by Ames test using TA98 and TA 100 and forward mutation test using TM 677 tester strain of S.typhimurium. No mutagenic..., stevioside (20 mg/ml) could not induce mutation in forward mutation using TM 677 with and without S-9 fractions from livers of the rats treated with PCB. However, the mutagenic activity of steviol (0.1-5 mg/ml) was detected only in forward mutation test usingPublication Metadata only Expression of foot and mouth disease virus nonstructural polyprotein 3ABC with inactive 3C pro in Escherichia coli(2011-11-01) Ladawan Sariya; Nattarat Thangthumniyom; Worawidh Wajjwalku; Wilairat Chumsing; Pongrama Ramasoota; Porntippa Lekcharoensuk; Mahidol University; Kasetsart UniversityNonstructural 3ABC protein of foot and mouth disease virus (FMDV) was widely used to differentiate vaccinated from natural FMDV-infected animals. 3ABC is a polyprotein which is auto-processed to 3A, three copies of 3B and 3C pro by 3C pro protease.... The 3ABC gene was cloned and expressed in Escherichia coli as native or mutated 3ABC (mu3ABC) forms. Cysteine residues 142 and 163 of the catalytic triad within the 3C pro of mu3ABC were changed to serine and glycine, respectively, to inhibit its