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Publication Open Access Exploring breast cancer data to find the risk factors for distant metastasis : register data, the national cancer institute of Thailand(2552) Auayporn, M; Montida Auayporn; Rojanavipart, P; Piangchan Rojanavipart; Viwatwongkasem, C; Chukiat Viwatwongkasem; Hancharoen, K; Kitiphong Hancharoen; Chaiwerawattana, A; Hancharoen, K; Kitiphong Hancharoen; มนต์ธิดา อวยพร; เพียงจันทร์ โรจนวิภาต; ชูเกียรติ วิวัฒน์วงศ์เกษม; กิติพงษ์ หาญเจริญA retrospective cohort of registered breast cancer patients at the National Cancer Institute of Thailand in 2004 was investigated to explore the association of prognostic factors with the probability of distant metastasis. The analysis was done... using binary and ordinal logistic regression models. There were 893 breast cancer patients included in a 3 year follow up study. During the study period 129(14.45%) were reported as metastasis cases, which the highest number of metastasis patientsPublication Open Access Impact of chest wall motion caused by respiration in adjuvant radiotherapy for postoperative breast cancer patients(2016) C. Lowanichkiattikul; M. Dhanachai; C. Sitathanee; S. Khachonkham; P. Khaothong; Mahidol University. Faculty of Medicine, Ramathibodi Hospitalradiotherapy treatment planning during December 2012 to November 2013 were included. Before scanning the radio-opaque wire was placed on the surface for breast and chest wall visualization on CT images, then the patient underwent three phases of CT scanning...To determine the chest wall movement of each patient during deep inspiratory breath hold (DIBH) and expiratory breath hold (EBH) in postoperative breast cancer patients. Postoperative breast cancer patients who underwent CT simulation for 3DPublication Open Access Adult stem cells and stem cell niche play important roles in primary mammary tumors(2009) WaraphanToniti; Mahidol University. Faculty of Veterinary Science. Department of Preclinic and Applied Animal SciencePublication Open Access Effect of Sex on Growth Curve, Production Performance and Carcass Quality of Cherry Valley Ducks(2018) Chanwit Kaewtapee; Kriengkrai Prahkarnkaeo; Chaiyapoom Bunchasak; Kasetsart University. Faculty of Agriculture. Department of Animal Science; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health, breast meat, feet and gizzard (P<0.05), whereas female tended to have greater (P=0.10) skin with subcutaneous fat. In conclusion, since the sex dimorphism of ducks showed significant effect on growth rate from 42 days of age, the separate sex growingItem Open Access Application of geospatial machine learning model for vegetation species image classification and above ground biomass estimation in upper and eastern gulf of Thailand mangrove forests(Mahidol University, 2021) Han, Tingting, 1995-; Poonperm Vardhanabindu; Paramita Punwong; Monthira YuttihamThe estimation of above-ground biomass in evergreen shrubs has traditionally been acquired from the ground measurements done by a labor-intensive method. Measuring such data requires diameter breast height (DBH), canopy cover, tree height, tree..., preventing the country from accurately assessing the total above-ground biomass. This research uses high-spatial-resolution data acquired from unmanned aerial vehicle (UAV) images to help measure the amount of above ground-biomass in forests. The assumptionPublication Open Access Effects of the three flavonoids; kaempferol, quercetin, and myricetin on Baby hamster kidney (BHK-21) cells and Human hepatocellular carcinoma cell (HepG2) cells proliferations and total Erk1/2 protein expression(2017) Sookruetai Boonmasawai; Arpron Leesombun; Kridsada Chaichoun; Jarupha Taowan; Ladawan Sariya; Orathai Thongjuy; Mahidol University. Faculty of Veterinary ScienceKaempferol, quercetin, and myricetin were regarded as the potential therapeutic flavonoids in several types of cancers. And the cancer cell proliferations that were directly induced by MAPK/Erk signaling pathway had been the important target of cancer treatment. Thus, this study was investigated the anti-proliferative effects of these three flavonoids together with total Erk1/2 protein expressions on Human hepatocellular carcinoma cell (HepG2) and Baby hamster kidney cells (BHK-2). The data showed the cytotoxic effects of 100 μM kaempferol, 5 μM myricetin and 50 μM quercetin on BHK-21 cells at 24h. After same incubation time, kaempferol (5 μM), myricetin (1 μM) and quercetin (1 μM) could significantly inhibit HepG2 cell proliferations. By western blot analysis, kaempferol and myricetin could not affect Total Erk1/2 proteins expression. But quercetin obviously suppressed total Erk1/2 protein expression in HepG2 cells at 24h. In conclusion, all three flavonoids had significant inhibitory effects on Hepatocellular carcinoma cells without cytotoxic effects on normal fibroblast. And inhibitory effects of quercetin in cancer cell proliferation related to total Erk1/2 protein reduction.Publication Open Access Determination of whole transcription profiles and specific pathways in invasive ductal breast carcinoma(2013-05-15) Pasra Arnutti; ภัสรา อาณัติ; Manas Kotepui; มนัส โคตรพุ้ย; Wichitra Asanprakit; Phaibul Punyarit; Porntip Chavalitshewinkoon-Petmitr; พรทิพย์ ชวลิตรชีวินกุล เพ็ชรมิตร; Talabporn Harnroongroj; ตรับพร หาญรุ่งโรจน์; Songsak Petmitr; ทรงศักดิ์ เพ็ชรมิตร; Songsak Petmitr; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Nutrition & Food Science.; Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine & Genetics.; Mahidol University. Faculty of Tropical Medicine. Department of Protozoology.Breast cancer is the most common cancer affecting women worldwide including Thailand. Whole transcription profiles of invasive ductal breast carcinoma (IDC) obtained by oligonucleotide microarray should lead to a better understanding... of the molecular basis of IDCs, allow for examination of specific markers for diagnosis, and provide novel targets for therapy. This study aimed to detect the whole transcript expression of approximately 35,000 target genes in Thai breast cancer patients, usingPublication Open Access Cancer-associated fibroblasts induce high mobility group box 1 and contribute to resistance to doxorubicin in breast cancer cells(2014) Kamolporn Amornsupak; Tonkla Insawang; Peti Thuwajit; Pornchai O-Charoenrat; Eccles, Suzanne A.; Chanitra Thuwajit; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of ImmunologyBackground: Cancer-associated fibroblasts and high mobility group box 1 (HMGB1) protein have been suggested to mediate cancer progression and chemotherapy resistance. The role of such fibroblasts in HMGB1 production in breast cancer is unclear.... This study aimed to investigate the effects of cancer-associated fibroblasts on HMGB1 expression in breast cancer cells and its role in chemotherapeutic response. Methods: Breast cancer-associated fibroblasts (BCFs) and non-tumor-associated fibroblastsPublication Open Access การพยาบาลผู้ป่วยหลังผ่าตัด Modified Radical Mastectomy and Immediate Breast & Nipple Reconstruction with Transverse Retus Abdominis Myocutaneous Flap(2547) สุวลักษณ์ วงศ์จรรโลงศิล; จิราภี สุนทรกุล ณ ชลบุรี; Suwaluck Wongjunlongsin; Jirapee Soonthornkul Na Chonburi; มหาวิทยาลัยมหิดล. คณะแพทยศาสตร์โรงพยาบาลรามาธิบดี. ภาควิชาพยาบาลศาสตร์Publication Open Access Aqueous extract of Thai medical Herbs (Phytoplex) Inhibits Cell Proliferation and Induces Apoptosis in human cervical cancer cell line (HeLa cells)(2018) Somjit Chaiwattanarungruengpaisan; Warunya Chakritbudsabong; Nattapat Rutjanavate; Rassameepen Phonarknguen; Ganokon Urkasemsin; Sasitorn Rungarunlert; สมจิตร ใช้วัฒนรุ่งเรืองไพศาล; วรัญญา ชาคริตบุษบง; ณัฐภัทร รุจจนเวท; รัศมีเพ็ญ โพธิ์นาคเงิน; กนกนอร เอื้อเกษมสิน; ศศิธร รุ่งอรุณเลิศ; Mahidol University. Faculty of Veterinary Science. The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE); Mahidol University. Faculty of Veterinary Science. Department of Preclinic and Applied Animal Science; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public HealthCervical cancer remains a leading cause of cancer mortality in females. Chemotherapy is important as it was a part of the main treatment for this type of cancer. However, multidrug resistance and serious side effects have been major problems in cervical cancer chemotherapy. Therefore, the search for new anticancer drugs from the native medicinal herbs, is very attractive. The combination extract of eight Thai medicinal herb recipes, namely Phytoplex, is a commercial product of The Government Pharmaceutical Organization, has anti-cancer effect on hepatocellular carcinoma cells (HepG2) in vitro. However, the anticancer effects and molecular mechanisms of Phytoplex on cervical cancer have not yet been studied. The aim of this study was to evaluate the inhibitory effect of Phytoplex on human cervical adenocarcinoma cells (HeLa cells) in vitro. HeLa cells were treated with low concentrations of Phytoplex (50, 100, 500, 1,000 μg/ml) and high concentrations of Phytoplex (2,000 and 5,000 μg/ml) compared with a positive control (0.1% mitomycin C) and a negative control (0 μg/ml of Phytoplex) for 24, 48 and 72 h. Then, cell viability was evaluated using an MTT assay. The activities of caspase-3 (apoptosis marker) and Ki-67 (proliferation marker)†were investigated using an immunofluorescence assay. At 24 h, lower concentrations of Phytoplex promoted cell viability, while higher concentrations inhibited cell viability (P < 0.05). In addition, the inhibitory effect of Phytoplex continuously increased from 24 to 72 h of incubation peroid at high concentrations of Phytoplex. Moreover, Phytoplex inhibited HeLa cell proliferation with an IC50 value of 1,972.43, 1,230.10 and 1,317.67 μg/ml at 24, 48 and 72 h, respectively. The activity of caspase-3 of HeLa cells treated with high concentration of Phytoplex seemed to be higher than of negative control (0 μg/ml). Activity of Ki-67 was higher in lower concentrations of Phytoplex, than that in higher concentrations of Phytoplex. Therefore, HeLa cell growth inhibition was dose-and time-dependent. The study suggested that high concentrations (2,000 and 5,000 μg/ml) of Phytoplex exerted inhibitory effect in HeLa cell growth by inducing apoptosis via activation of caspase-3.