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Publication Open Access Responses of primary human nasal epithelial cells to EDIII-DENV stimulation: the first step to intranasal dengue vaccination(2016) Nattika Nantachit; Panya Sunintaboon; Sukathida Ubol; Mahidol University. Faculty of Science. Department of Microbiologyof vaccination. The tested immunogen was the domain III of dengue serotype-3 E protein (EDIII-D3) loaded into trimethyl chitosan nanoparticles (EDIII-D3 TMC NPs). The primary human nasal epithelial cells, HNEpCs, were used as an in vitro model for nasal... responses. Results: At tested concentrations, EDIII-D3 TMC NPs not only exerted no detectable toxicity toward HNEpC cultures but also efficiently delivered EDIII-D3 immunogens into HNEpCs. Moreover, HNEpCs quickly and strongly produced proinflammatoryPublication Open Access Dengue virus infection induces expansion of a CD14(+)CD16(+) monocyte population that stimulates plasmablast differentiation.(2014-07-09) Kwissa Marcin; Nakaya, Helder I.; Nattawat Onlamoon; Wrammert Jens; Villinger Francois; Perng, Guey Chuen; Sutee Yoksan; Kovit Pattanapanyasat; Kulkanya Chokephaibulkit; Ahmed Rafi; Pulendran Bali; Mahidol University. Faculty of Medicine Siriraj Hospital. Insitute of Molecular Biosciences; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Pediatricsare generated, we used a systems biological approach to analyze immune responses to dengue in humans. Transcriptomic analysis of whole blood revealed that genes encoding proinflammatory mediators and type I interferon-related proteins were associated with high...Dengue virus (DENV) infection induces the expansion of plasmablasts, which produce antibodies that can neutralize DENV but also enhance disease upon secondary infection with another DENV serotype. To understand how these immune responsesPublication Open Access iNKT cells and their potential lipid ligands during viral infection.(2015-07-24) Anunya Opasawatchai; อนัญญา โอภาสวัตชัย; Ponpan Matangkasombut; Ponpan Matangkasombut; Mahidol University. Faculty of Dentistry; Mahidol University. Faculty of Science. Department of Microbiology. Several viruses, such as dengue virus, induce CD1d upregulation, which correlates with iNKT cell activation. In contrast, herpes simplex virus type 1 (HSV-1), human immunodeficiency virus (HIV), Epstein-Barr virus, and human papilloma virus promote CD1d... downregulation as a strategy to evade iNKT cell recognition. These observations suggest the participation of a CD1d-dependent process in the activation of iNKT cells in response to viral infection. Endogenous lipid ligands, including phospholipids as wellPublication Open Access Naturally-acquired cellular immune response against Plasmodium vivax merozoite surface protein-1 paralog antigen(2015) Siriruk Changrob; Chaniya Leepiyasakulchai; Eun-Taek Hancorresponding; Takafumi Tsuboi; Patchanee Chootong; Yang Cheng; Chae Seung Lim; Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied Technologywere detected during P. vivax infection and the antibody inhibited PvMSP1P-erythrocyte binding. Recombinant PvMSP1P antigen results in production of a significant Th1 cytokine response in immunized mice. The present study was performed to characterize... response against PvMSP1P-19 and PvDBPII protein antigen, PBMCs from subjects who had recovered from P. vivax infection 8-10 weeks prior to the study were obtained for lymphocyte proliferation assay. Cytokine-producing cells were analysed by flow cytometryPublication Open Access Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation.(2014-07-10) Salunya Tancharoen; ศรัณยา ตันเจริญ; Tassanee Tengrungsun; ทัศนีย์ เต็งรังสรรค์; Theeralaksna Suddhasthira; ธีรลักษณ์ สุทธเสถียร; Kikuchi, Kiyoshi; Vechvongvan, Nuttavun; Tokuda, Masayuki; Maruyama, Ikuro; Mahidol University. Faculty of Dentistry. Department of Pharmacology; Mahidol University. Faculty of Dentistry. Department of Advanced General Dentistry; Mahidol University. Faculty of Dentistry. Department of Pharmacologyin human pulpitis tissue. LPS stimulated OLC-1 cells produced HMGB1 in a dose-dependent manner through RAGE. HMGB1 translocation towards the cytoplasm and secretion from OLC-1 in response to LPS was inhibited by TPCA-1, an inhibitor of NF-κB activation..., stromal pulp fibroblasts-like cells, and endothelial-like cell lining human pulpitis tissue. Strong cytoplasmic HMGB1 immunoreactivity was noted in odontoblasts, whereas nuclear HMGB1 immunoreactivity was seen in stromal pulp fibroblasts-like cellsPublication Open Access The involvement of microglial cells in japanese encephalitis infections(2012) Thananya Thongtan; Chutima Thepparit; Smith, Duncan R.; Mahidol University. Faculty of Medicine. Department of Microbiology.; Mahidol University. Institute of Molecular Biosciences. Molecular Pathology Laboratory; Mahidol University. Center for Emerging and Neglected Infectious DiseasesDespite the availability of effective vaccines, Japanese encephalitis virus (JEV) infections remain a leading cause of encephalitis in many Asian countries. The virus is transmitted to humans by Culex mosquitoes, and, while the majority of human... infections are asymptomatic, up to 30% of JE cases admitted to hospital die and 50% of the survivors suffer from neurological sequelae. Microglia are brain-resident macrophages that play key roles in both the innate and adaptive immune responses in the CNSPublication Open Access Third-stage Gnathostoma spinigerum larva excretory secretory antigens modulate function of Fc gamma receptor I-mediated monocytes in peripheral blood mononuclear cell culture(2016) Surachet Benjathummarak; Ratchanok Kumsiri; Supaporn Nuamtanong; Thareerat Kalambaheti; Jitra Waikagul; Nareerat Viseshakul; Yaowapa Maneerat; Mahidol University. Faculty of Tropical Medicine. Department of Tropical PathologyBackground: Third (infective)-stage Gnathostoma spinigerum larvae (L3) mainly cause human gnathostomiasis. G. spinigerum L3 migrate throughout the subcutaneous tissues, vital organs, and central nervous system and can cause various pathogenesis...). This study investigated whether ES antigen (ESA) from G. spinigerum L3 affects monocyte functions. Results: Cultures of normal peripheral blood mononuclear cells (PBMC) separated from healthy buffy coats were used as a human immune cell model. ESAPublication Open Access Common TLR1 genetic variation is not associated with death from melioidosis, a common cause of sepsis in rural Thailand(2014-01-02) Narisara Chantratita; นริศรา จันทราทิตย์; Sarunporn Tandhavanant; ศรัณย์พร ตัณฑวนันท์; Myers, Nicolle D.; Wirongrong Chierakul; วิรงค์รอง เจียรกุล; Vanaporn Wuthiekanun; วรรณพร วุฒิเอกอนันต์; Weera Mahavanakul; Direk Limmathurotsakul; ดิเรก ลิ้มมธุรสกุล; Peacock, Sharon J.; West, T. Eoin; Narisara Chantratita; Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygieneand rs4833095. The variants were in high linkage disequilibrium but neither variant was associated with organ failure or in-hospital death. In 300 healthy Thai individuals we further tested the association of TLR1 variation with ex vivo blood responses... to Pam3CSK4, a TLR1 agonist. Neither variant was robustly associated with blood cytokine responses induced by Pam3CSK4. We identified additional common variation in TLR1 by searching public databases and the published literature and screened threePublication Open Access A comparative study of natural immune responses against Plasmodium vivax C-terminal merozoite surface protein-1 (PvMSP-1) and apical membrane antigen-1 (PvAMA-1) in two endemic settings(2015-08-06) Hui Xia; Qiang Fang; Kulachart Jangpatarapongsa; Tao Zhiyong; Liwang Cui; Baiqing Li; Rachanee Udomsangpetch; Mahidol University. Faculty of Medical Technology. Center for Research and Innovation; Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied TechnologyThe mechanisms of cellular and humoral immune responses against P. vivax parasite remain poorly understood. Several malaria immunological studies have been conducted in endemic regions where both P. falciparum and P. vivax parasites co-exist.... © 2015, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved.Publication Open Access Thermoregulatory correlates of nausea in rats and musk shrews(2014) Sukonthar Ngampramuan; Matteo Cerri; Flavia Del Vecchio; Corrigan, Joshua J; Amornrat Kamphee; Dragic, Alexander S; Rudd, John A; Romanovsky, Andrej A; Eugene Nalivaiko; Mahidol University. Institute of Molecular Biosciencesby the presence of retching and vomiting. In this model, sickness was also accompanied by marked hypothermia (~2°C). Like in rats, the hypothermic response was preceded by transient tail-skin vasodilation. In conclusion, motion sickness is accompanied