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- ItemUltrastructure of follicle cell and its steroid secretion in Rana tigerina(Mahidol University. Mahidol University Library and Knowledge Center, 1997) Tarinee Sawatpanich; Prapee Sretarugsa; Prasert Sobhon; Jittipan Chavadej; Maleeya KruatrachueIn order to study the ultrastructure of follicle cells as well as the numbers surrounding the various stages of oocytes, the defolliculated oocytes were fixed in 2.5% glutaraldehyde in 0.05 M Millonig buffer for 2-4 hours, intact follicles werefixed in 4% glutaraldehhde, 2% paraformaldehyde in 0.05 M Millonig buffer. Defolliculated oocytes were processed for examination by scanning electron microscopy, and the number of follicle cells were counted, whereas the intact oocytes were prepared for examination by conventional transmission electron microscopy. In addition, the follicles of all stages were measured for the levels of estradiol and progesterone by radioimmunoassay. The follicle cells have flattened and oval shape. The surface close to the theca layer exhibits short microvilli while there are numerous slender processes protruding from the lateral and ventral surfaces. Some ventral processes traverse the vitelline coat to adhere to the oocytes membranes. Follicle cells are connected to each other by desmosomes at the contacts between their thin cytoplasmic processes. Follicle cells from stage I and II oocytes contain short RER, mitochondria with few cristae, and free ribosomes. Their nuclei contain mostly euchromatin with only thin rim of heterochromatin along the nuclear envelopes. The number of mitochondria with elaborate cristae, RER, free ribosomes, SER, Golgi complex, coated vesicle increase in follicle cells from stage III oocytes. The ultrastructures of follicle cells from stages IV,V and VI oocytes resemble those from stage III oocyte. The number of follicle cells increase with the oocyte growth. The oocytes with diameters range 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5 and 1.6 mm are surrounded by 9430 ± 572, 13126 ± 670, 17185 ± 984, 18571 ± 1356, 19730 ± 3121, 21049 ± 3019, 25160 ± 406, 28668 ± 1075, 36566 ± 1152 follicle cells, respectively. Both control and frog pituitary-hormone-treated follicles were cultured for six hours and the amount of estradiol and progesterone present in both the medium and follicles was determined by radioimmunoassay. Previtellogenic (stage I and II) follicles secreted very low levels of these two steroids in both presence and absence of frog pituitary hormone. Vitellogenic follicles were active in secreting estradiol. The highest level of estradiol was secreted by stage IV follicle (53.5 pg/ follicle). In follicles larger than stage IV, estradiol secretion decreased as follicular size increased. In contrast, full-grown follicle secreted much-more progesterone (52.7 pg/ follicle) than previtellogenic and vitellogenic follicles.
- ItemBiology of black flies (Simulium) at Doi Inthanon National Park, Northern Thailand(Mahidol University. Mahidol University Library and Knowledge Center, 1998) Suwannee Phayuhasena; Chaliow Kuvangkadilok; Visut BaimaiIn the present study, morphotaxonomy, distribution and cytogenetics of the black fly larvae were studied. The larvae of black flies were collected from various localities at 400-2,500 meters above sea level of Doi Inthanon National Park, Chiangmai province. A total of 17 species including 13 known species, three unnamed species and one new species, were identified and placed into several species-groups within 6 subgenera of the genus Simulium Latreille s.1. Their distribution seems to correlate with altitudes. Different species were found at different altitudes with various habitats. The six species of Simulium, S. caudisclerum, S. feuerborni, S. fenestratum, S. nakhonense, S. rufibasis and S species G, cytologically studied here have three pairs of chromosomes (2N=6) and their arrangement are species-specific. A total of six paracentric inversions distributed in five chromosome arms have been detected in natural populations of S. feuerborni. Significant deviation from Hardy-Weinberg equilibrium has been observed in inversion IIIL-1. There is no indication of sex linkage associated with any inversion sequence in these populations. Study of C-banding patterns of larval salivary gland polytene chromosomes of the above species using C-banding technique revealed interspecific and intraspecific differences. These differences were in respect to the amount and distribution of constitutive heterochromatin. Moreover, band 84B2 of the chromosome arm IIIL of S. nakhonense is sex-linked C+ heterochromatin which may mark the X or Y chromosome. It is possible that the sex determining system in S. nakhonense is heterogametic in female (XY) and homogametic in male (XX).
- ItemA design and development of the student registration and evaluation system using object-oriented technology and relational database(Mahidol University. Mahidol University Library and Knowledge Center, 1999) Sirirat Glinhom; Thanakorn Uan-on; Wanchai Rivepiboon; Choumchok NamsrisakulratThe purposes of this research were to design and develop a student registration and evaluation system (SRE), which is a primary information system in academics. Boromarajonani College of Nursing Ratchaburi (BCNR) was selected to be the model of study. The research used the Object-Oriented Software Engineering (OOSE) of Ivar Jacobson, an Actor-Use case driven approach. By this concept, developer and user have the same vision of the system. The research is divided into 5 parts, Accessing the SRE system, Data Initialization, Registration, Evaluation and Reports. The first part is users and their permission verification. The second part, data initialization into the relational database, uses MS Access 97 as the database management system. The third part, registration, adds and drops courses. The next part evaluation, is for the instructor use. Finally the reports generation creates student and class reports. The application software was constructed in Visual Basic 6.0, which supports Object-Oriented Programming and Graphic User Interface. The SRE system runs on the standalone by installing the application program and database file on the same PC. Also, it runs on LAN by installing the application program on the client(s) and database file on the server. Officers, instructors and students did system testing. They concluded that the SRE system was useful in operation of student registration and evaluation. Also, it reduced bottleneck problems of the registrar because each user group could perform tasks and request information by themselves. The SRE system facilitates registration operation and management and evaluation transactions of the organization. Furthermore, this research will serve as a guideline in future system development with Object-Oriented technology
- ItemComparison of ELISA and DOT blot hybridization with agarose gel electrophoresis for rapid detection of pcr products from mycobacterium tuberculosis(Mahidol University. Mahidol University Library and Knowledge Center, 1999) Sarawut Suttirat; Unchalee Tansuphasiri; Somsak Rienthong
- ItemEffects of chest physiotherapy with and without inspiratory muscle training in moderate risk patients undergoing upper abdominal surgery(Mahidol University. Mahidol University Library and Knowledge Center, 1998) Saowanee Woravutrangkul, 1973; Phongthara Vichitvejpaisal; Suwannee Jarungjitaree; Somchart Maneenoy
- ItemIn vivo variability of viral protease from HIV-1 subtype E infected Thai patients treated with reverse transcriptase inhibitors(Mahidol University. Mahidol University Library and Knowledge Center, 1999) Rerngwit Boonyom; Sakol Panyim; Wichet Leelamanit; Somchai Pongpattanakitshote
- ItemRapid detection of vibrio parahaemolyticus in seafood by using DNA hybridization assay and PCR(Mahidol University. Mahidol University Library and Knowledge Center, 1998) Siriwan Kachornchaiyakul; Orasa Suthienkul; Kanokrat Siripanichgon; Nednapis Tirawanchai
- ItemP53 expression and steroid receptor status in breast cancer(Mahidol University. Mahidol University Library and Knowledge Center, 1998) Oranan Preechanont; Suntaree Apibal; Sansanee Wongwaisayawan; Budsaba Rerkamnuaychoke; Pongsak Wannakrairot; Sunanta Chariyalertsak
- ItemMHC class II alleles and immune response to hepatitis B vaccination in Thais(Mahidol University. Mahidol University Library and Knowledge Center, 1998) Jongkol Akahat; Pimol Chiewsilp; Urusa Theppisai; Tasanee Mongkolsuk; Kanchana Sujirachato
- ItemA study of complex chemical oscillations in the pseudo-closed system of belousov-zhabotinsky reaction(Mahidol University. Mahidol University Library and Knowledge Center, 1997) Methasit Pornprompanya; Prapin Wilairat; Orapin Rangsiman; Preedeeporn Limcharoen; Waret VeerasaiIn the investigation of the complex oscillations of intermediates bromide and cerium ions by potentiometric method in a pseudo-closed system of cerium(IV)-catalyzed Belousov-Zhabotinsky reaction at a constant temperature of 30.0+0.1C, malonic acid and cerium(IV) concentrations were 0.44 and 0.00133 M, respectively, and bromate concentration was increased: 0.093, 0.105 and 0.118 M in 1.00 M sulfuric acid. A number of transient phenomena including mixed-mode (MM) and intermittency(I) oscillations were observed in between two series of simple oscillations. When the sulfuric acid concentration was reduced from 1.00 to 0.500 M, the complex transient oscillations were no longer observed. There were only damped simple oscillations with 1-3 cycles of period-doubled oscillations. The induction period was independent of the bromate concentration employed in this study, but it decreased significantly when the sulfuric acid concentration was reduced from 1.00 to 0.500 M. The complex oscillations could only be reproduced in a qualitative manner as they have shown to be very sensitive to the concentrations of the major reactants in the reaction.