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Publication Metadata only Immunogenicity of liposome-associated and refined antigen oral cholera vaccines in Thai volunteers(1998-04-01) Wanpen Chaicumpa; Manas Chongsa-Nguan; Thareerat Kalambaheti; Polrat Wilairatana; Potjanee Srimanote; Yuvadee Makakunkijcharoen; Sornchai Looareesuwan; Yuwaporn Sakolvaree; Mahidol UniversityA mixture of Vibrio cholerae antigens made up of crude fimbrial extract, lipopoly-saccharide and procholeragenoid was administered orally to Thai volunteers either as free antigen or associated with liposomes. All vaccinees and controls were... administered in three doses given at 14 day intervals. Nine volunteers received liposome-associated vaccine and seven received free vaccine. Liposomes without antigens were given to eight volunteers and seven volunteers received 5% NaHCO3solution alone. BothPublication Metadata only CpG DNA, liposome and refined antigen oral cholera vaccine(2003-12-01) Ratree Leelawongtawon; Srinuan Somroop; Urai Chaisri; Pongsri Tongtawe; Manas Chongsa-nguan; Thareerat Kalambaheti; Pramuan Tapchaisri; Sathit Pichyangkul; Yuwaporn Sakolvaree; Hisao Kurazono; Hideo Hayashi; Wanpen Chaicumpa; Mahidol University; Thammasat University; Armed Forces Research Institute of Medical Sciences, Thailand; Okayama University; University of TsukubaAn oral cholera vaccine made up of three Vibrio cholerae antigens, i.e. lipopolysaccharide (LPS), recombinant toxin co-regulated pili (rTcpA) and heat-treated cholera toxin (H-CT) has been developed in six different formulations. Eight-week-old... Wistar rats were divided into nine groups and immunized as follows: the first group received the oral vaccine 1 consisting of the three antigens (LPS, rTcpA and H-CT) associated with a liposome (L) and bacterial CpG-DNA (ODN#1826). The rats of groups 2Publication Metadata only Trichinella spiralis-specific monoclonal antibodies and affinity-purified antigen-based diagnosis(2000-03-01) Potjanee Srimanote; Wannaporn Ittiprasert; Banguorn Sermsart; Urai Chaisri; Pakpimol Mahannop; Yuwaporn Sakolvaree; Pramuan Tapchaisri; Wanchai Maleewong; Hisao Kurazono; Hideo Hayashi; Wanpen Chaicumpa; Mahidol University; Khon Kaen University; University of TsukubaHybridomas secreting monoclonal antibodies (MAbs) to Trichinella spiralis were produced. Myeloma cells were fused with splenocytes of a mouse immunized with excretory-secretory (E-S) antigen of infective larvae. A large percentage of growing hybrids... secreted antibodies crossreactive to many of 23 heterologous parasites tested. Only 6 monoclones (designated 3F2, 5D1, 10F6, 11E4, 13D6 and 14D11) secreted MAbs specific to the E-S antigen and/or a crude extract (CE) of T. spiralis infective larvae. The 6Publication Metadata only Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis(1991-01-01) Chamnong Nopparatana; Prasert Setasuban; Wanpen Chaicumpa; Pramuan Tapchaisri; Mahidol UniversitySpecific antigen of G. spinigerum which has been shown to be a protein with a relative mol. wt of 24,000 (24k) was prepared from the advanced third-stage larvae (L3) obtained from the livers of naturally infected eels. The L3 were ground... and extracted with water. Purification procedures involved gel filtration, chromatofocussing and anion exchange column chromatographies, while characterization of the specific antigen was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresisPublication Metadata only Specific antigen of Gnathostoma spinigerum for immunodiagnosis of human gnathostomiasis(1991-01-01) Pramuan Tapchaisri; Chamnong Nopparatana; Wanpen Chaicumpa; Prasert Setasuban; Mahidol Universityelectrophoresis (SDS-PAGE) and Western blot analysis for their reactivities against somatic extract of Gnathostoma spinigerum third-stage larvae (L3). It was found that the L3 extract was highly complex consisting of more than 20 antigenic components, a few... of which gave reactions with sera from the healthy controls. Extensive cross-reactions of the parasite's antigen with sera from patients with other parasitic infections occurred. A specific antigen of G. spinigerum with a mol. wt of 24,000 (24k) was foundPublication Metadata only Specific monoclonal antibodies to Strongyloides stercoralis: A potential diagnostic reagent for strongyloidiasis(2002-12-01) Piyanan Taweethavonsawat; Wanpen Chaicumpa; Urai Chaisri; Usa Chuenbal; Yuwaporn Sakolvaree; Pramuan Tapchaisri; Thitima Wongsaroj; Thammasat University; Mahidol UniversityIn this study, specific hybridomas secreting monoclonal anti-bodies (MAb) to antigen of Strongyloldes stercoralis filariform larvae were produced. Specific epitopes targeted by the MAb were protein in nature and located in situ in the internal... content of the filariform larvae of the parasite but not in the esophagus. The MAb reacted to the homologous antigen in an indirect ELISA but did not reveal any reaction to the SDS-PAGE separated-homologous antigen in a Western blot analysis (WBPublication Metadata only Towards a suitable antigen for diagnosis of Gnathostoma spinigerum infection(1992-01-01) Chammong Nopparatana; Wanpen Chaicumpa; Pramuan Tapchaisri; Prasert Setasuban; Yuwaporn Ruangkunaporn; Mahidol UniversityNopparatana C., Chaicumpa W., Tapchaisri P., Setasuban P. and Ruangkunaporn Y. 1992. Towards a suitable antigen for diagnosis of Gnathostoma spinigerum infection. International Journal for Parasitology 22: 1151-1156. Advanced third-stage larvae of G... brilliant blue staining while their antigenicities were studied by Western blot analysis using serum of a patient with parasitologically confirmed gnathostomiasis. Distilled water was found to be the best extraction solution in solubilizing proteinsPublication Metadata only Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus(1991-01-01) Isna Indrawati; Wanpen Chaicumpa; Prasert Setasuban; Yuwaporn Ruangkunaporn; Mahidol UniversityAdult Paragonimus heterotremus were recovered from the lungs and pleural cavity of cats orally infected with metacercariae. The worms were ground and extracted with distilled water. The soluble crude antigen (CA) contained about 40% proteins which...). The sensitivity and specificity of the assay when the F1was used as the antigen were 100%. Western blot analysis revealed that specific antigen of P. heterotremus was a non-protein component of Mr35 kDa. © 1991.Publication Metadata only Affinity purified oval antigen for diagnosis of Opisthorchiasis Viverrini(2001-12-01) Thitima Wongsaroj; Yuwaporn Sakolvaree; Wanpen Chaicumpa; Wanchai Maleewong; Viroj Kitikoon; Pramuan Tapchaisri; Manas Chongsa-nguan; John H. Cross; Mahidol UniversityMonoclonal antibodies (MAb) were raised against an oval antigen of the liver fluke Opisthorchis viverrini which is the causative agent of a parasitosis, i.e. opisthorchiasis in Thailand. The antibodies were used in an affinity column to purify the O.... viverrini oval antigen from a crude extract of adult parasites by chromatography. The oval antigen was then used in a membrane (dot) ELISA for detecting antibodies in serum samples of parasitologically confirmed Opisthorchis viverrini infected individualsPublication Metadata only Immunodiagnosis of human trichinellosis using excretory-secretory (ES) antigen(1992-01-01) Wanpen Chaicumpa; Prasert Setasuban; Faculty of Public Health; Mahidol University; Chiang Mai University-free medium for 18 h. The ES antigen obtained from the culture medium was used in an enzyme-linked immunosorbent assay (ELISA) for detecting IgG antibodies to T. spiralis in serum samples collected from three groups of individuals. The individuals... was also 100% when performed on sera of group 1 collected at days 57 and 120 after infection. Sera collected earlier (day 23) and those collected 700 days after infection had negligible reactivity. Thus IgG-ELISA using ES antigen of the L1was useful