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Publication Metadata only Enzyme-linked immunosorbent assay for detection of antibody to Gnathostoma antigen in patients with intermittent cutaneous migratory swelling(1986-08-06) A. Dharmkrong-At; S. Migasena; P. Suntharasamai; D. Bunnag; R. Priwan; S. Sirisinha; Mahidol UniversityA sensitive enzyme-linked immunosorbent assay is described for the detection of immunoglobulin G antibody to Gnathostoma antigen in the sera of patients with intermittent cutaneous migratory swelling who were suspected to being infected... by the tissue nematode Gnathostoma spinigerum. The antigen used was a crude somatic aqueous extract of the third larval stage obtained from naturally infected eels. The 1:320 dilution of all sera from 46 patients gave enzyme-linked immunosorbent assay valuesPublication Metadata only Serodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody(2005-06-01) Charin Thepthai; Saijai Smithtikarn; Montana Suksuwan; Sirirurg Songsivilai; Tararaj Dharakul; Mahidol University; Thailand Ministry of Public Healthcompetitive enzyme-linked immunosorbent assay (ELISA) using a lipopolysaccharide-specific monoclonal antibody was developed. The assay provides high specificity, based on a previously described monoclonal antibody to a specific epitope... on the lipopolysaccharide (LPS) of B. pseudomallei. The assay sensitivity of 96.0% and specificity of 100% were achieved at a cutoff value of 50% inhibition in human culture-proven melioidosis cases. An optimal cutoff value of 65% inhibition for sera from a melioidosisPublication Metadata only Rapid detection of Salmonella enterica serovar Choleraesuis in blood cultures by a dot blot enzyme-linked immunosorbent assay(2000-12-01) K. Janyapoon; S. Korbsrisate; H. Thamapa; S. Thongmin; S. Kanjanahareutai; N. Wongpredee; S. Sarasombath; Mahidol UniversityA dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella enterica serovar Choleraesuis in blood cultures. This system was applied.... In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.Publication Metadata only Enzyme-linked immunosorbent assay based on monoclonal and polyclonal antibodies for the detection of Entamoeba histolytica antigens in faecal specimens(1992-01-01) Ratri Wonsit; Nitaya Thammapalerd; Savanat Tharavanij; Prayong Radomyos; Danai Bunnag; Mahidol UniversityA murine monoclonal antibody (MAb) (Eh208C2-2) raised against crude lysate of the pathogenic HM- 1:IMSS strain of Entamoeba histolytica was used in an enzyme-linked immunosorbent assay for the detection of E. histolytica antigens in faecal specimens.... The detection limit of the assay was 110 and 280 amoebae/ml of the HM-1:IMSS and HK-9 strains in phosphate-buffered saline, respectively. The assay was applied to single stool samples from 3 groups of individuals comprising 40 patients whose stools were positivePublication Metadata only HIV-1 subtyping using gag/env heteroduplex mobility assay and peptide enzyme-linked immunosorbent assay(2005-06-01) Wantanee Simparak; Uraiwan Kositanont; Ruengpung Sutthent; Punneeporn Wasinrapee; Thanyanan Chaowanachan; Chantapong Wasi; Mahidol University; Centers for Disease Control (CDC), Thailand Field Stationyears. During 1997-1998, 227 blood samples were collected from HIV-1 infected individuals consisting of 92 mothers, 35 children and 100 IDUs. The blood samples were subtyped by heteroduplex mobility assay (HMA) and peptide enzyme-linked immunosorbent... assay (PEIA). Using gag and env HMA, CRF01Æ and subtype B′ accounted for 96-97% and 3-4% of both the mothers and the children, respectively. In the IDU group, 10% of the plasma samples could only be performed by gag HMA and gave the result as CRF01Æ. CRFPublication Metadata only Evaluation of biotin-streptavidin enzyme-linked immunosorbent assay for detection of genital herpes simplex virus infection.(1987-12-01) C. Yoosook; P. Rimdusit; W. Chantratita; P. Leechanachai; P. Bhattarakosol; Mahidol UniversityA biotin-streptavidin enzyme-linked immunosorbent assay (B-SA ELISA) was evaluated for detection of herpes simplex virus (HSV) in clinical specimens which were cervico-vaginal swabs from 205 asymptomatic women and swabs from the genital lesionsPublication Metadata only Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis(1990-06-18) M. Kunakorn; P. Boonma; K. Khupulsup; B. Petchclai; Mahidol Universityin the endemic areas. An enzyme-linked immunosorbent assay (ELISA) for IgM antibody is an attractive alternative. An indirect ELISA for IgM antibody (IgM ELISA) and an IgM antibody capture ELISA for melioidosis were developed. Both tests, together with IHA, werePublication Metadata only Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis(2007-01-01) Narisara Chantratita; Vanaporn Wuthiekanun; Aunchalee Thanwisai; Direk Limmathurotsakul; Allen C. Cheng; Wirongrong Chierakul; Nicholas P.J. Day; Sharon J. Peacock; Mahidol University; Menzies School of Health Research; Nuffield Department of Clinical MedicineFive enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed... for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%). Copyright © 2007, AmericanPublication Metadata only Enzyme-linked immunosorbent assay for detection of Salmonella typhi protein antigen(1987-03-17) H. Appassakij; N. Bunchuin; S. Sarasombath; B. Rungpitarangsi; S. Manatsathit; P. Komolpit; T. Sukosol; Mahidol UniversityA double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was designed for the detection of Salmonella typhi protein antigen. The optimal concentration of antibody for coating the plate was found to be 5 μg/ml. The optimum conditions... for antibody coating and antigen and conjugate incubation were 37°C for 3 h, 37°C for 2 h, and 4° C overnight, respectively. The enzyme-substrate reaction was allowed to take place at 30°C for 1 h. The established ELISA was found to be reproduciblePublication Metadata only Detection of plasmodium sporozoites in anopheles mosquitoes using an enzyme-linked immunosorbent assay(2021-09-01) Chalermpon Kumpitak; Wang Nguitragool; Liwang Cui; Jetsumon Sattabongkot; Sirasate Bantuchai; Faculty of Tropical Medicine, Mahidol University; Morsani College of Medicineof sporozoites in the mosquito salivary glands thus defines mosquito infectiousness. To determine whether an Anopheles mosquito carries Plasmodium sporozoites, the enzyme-linked immunosorbent assay (ELISA) method has been the standard tool to detect