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Item Metadata only Quantitative analysis of Lactobacillus and Enterococcus Faecalis in irreversible pulpitis and pulp necrosis of primary teeth(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Wananong Watcharakirin; Kemthong Mitrakul; Kadkao VongsawanThe objectives of this study were to quantitatively analyze and compare Lactobacillus and Enterococcus faecalis levels in infectious root canal of primary teeth between irreversible pulpitis and pulp necrosis and correlations with history of pain....017). In conclusion, Lactobacillus and Enterococcus faecalis were detected in infectious root canal of primary teeth, and Lactobacillus was correlated with history of pain in both irreversible pulpitis and pulp necrosis.Publication Metadata only Optimization of cryoprotectants for freeze-dried potential probiotic Enterococcus faecalis and evaluation of its storage stability(2021-01-01) Chonticha Romyasamit; Phanvasri Saengsuwan; Panadda Boonserm; Benjamas Thamjarongwong; Kamonnut Singkhamanan; Faculty of Medicine, Prince of Songkia University; Institute of Molecular Biosciences, Mahidol Universitypreparation. Recently, Enterococcus spp. such as Enterococcus faecalis and E. faecium have been used as probiotics for human or animal health promotion. We aimed to determine a suitable protectant to protect the viability and pathogen inhibition potential of E.... faecalis probiotic strains during freeze-drying and subsequent storage. E. faecalis probiotic strains were lyophilized in the presence of glucose, fructose, sucrose, skim milk, and distilled water. The lyophilized vials were stored at 4 °C for up to 30 daysPublication Metadata only Enterococcus faecalis enhances cell proliferation through hydrogen peroxide-mediated epidermal growth factor receptor activation(2012-10-01) Kanitsak Boonanantanasarn; Ann Lindley Gill; YoonSing Yap; Vijayvel Jayaprakash; Maureen A. Sullivan; Steven R. Gill; University of Rochester School of Medicine and Dentistry; University at Buffalo, State University of New York; Mahidol University; Roswell Park Cancer InstituteEnterococcus faecalis is a member of the intestinal and oral microbiota that may affect the etiology of colorectal and oral cancers. The mechanisms by which E. faecalis may contribute to the initiation and progression of these cancers remain... uncertain. Epidermal growth factor receptor (EGFR) signaling is postulated to play a crucial role in oral carcinogenesis. A link between E. faecalis and EGFR signaling in oral cancer has not been elucidated. The present study aimed to evaluateItem Metadata only Cannabidiol Demonstrates Remarkable Efficacy in Treating Multidrug-Resistant Enterococcus Faecalis Infections In Vitro and In Vivo(2024-09-01) Hongsing P.; Ngamwongsatit N.; Kongart C.; Nuiden N.; Phairoh K.; Wannigama D.L.; Hongsing P.; Mahidol UniversityThe growing prevalence of multidrug-resistant Enterococcus faecalis infection has become a global concern. There is a demand for alternative antibacterial agents, such as herbal alternatives, such as Cannabidiol, that are cost-effective, non-toxic... and efficient. This study investigates the Minimum Inhibitory Concentration (MIC) for planktonic cells and the Minimum Biofilm Eradication Concentration (MBEC) for biofilm formation in multidrug-resistance isolates of E. faecalis isolates, particularly focusingPublication Metadata only Transferable plasmid-mediated resistance to linezolid due to cfr in a human clinical isolate of Enterococcus faecalis(2012-07-01) Lorena Diaz; Pattarachai Kiratisin; Rodrigo E. Mendes; Diana Panesso; Kavindra V. Singh; Cesar A. Arias; University of Texas Medical School at Houston; Universidad El Bosque; Mahidol University; JMI Laboratories Incorporated, it has been identified in Staphylococcus aureus from human clinical infections, including in an outbreak of methicillin-resistant S. aureus. In enterococci, cfr has been described in an animal isolate of Enterococcus faecalis from China. Here, we report... laboratory strain of E. faecalis (OG1RF) but not to Enterococcus faecium or S. aureus. The cfr gene was flanked by IS256-like sequences both upstream and downstream. This is the first characterization of the potential horizontal transferability of the cfrItem Metadata only Effects of Postbiotic from Bacteriocin-Like Inhibitory Substance Producing Enterococcus faecalis on Toxigenic Clostridioides difficile(2023-07-01) Luenglusontigit P.; Sathapondecha P.; Saengsuwan P.; Surachat K.; Boonserm P.; Singkhamanan K.; Mahidol UniversityObjective: To determine the activities of postbiotics, prepared from bacteriocin-like producing Enterococcus faecalis (E. faecalis) against Clostridioides difficile (C. difficile) and its spores, and to assess the safety of postbiotics in vivo.... Material and Methods: Bacteriocin production of E. faecalis PK1201 was screened by proteolytic enzyme treatment, and bacteriocin-encoding genes were characterized by whole genome sequencing. A postbiotic of E. faecalis PK1201 was prepared using neutralizedPublication Metadata only In vitro assessment of Enterococcus faecalis MTC 1032 as the potential probiotic in food supplements(2018-07-01) Sirikhwan Tinrat; Piyatip Khuntayaporn; Krit Thirapanmethee; Mullika Traidej Chomnawang; King Mongkut's University of Technology North Bangkok; Mahidol Universitysequence revealed that this strain was Enterococcus faecalis. Interestingly, this strain effectively inhibited a variety of pathogens by acid and bacteriocin production and was bile-tolerant, able to survive under acidic condition. In the safety assessments..., E. faecalis MTC 1032 could adhere to host epithelial cells and evidently inhibited pathogenic cell adhesion as demonstrated by cell reduction over time of E. coli ATCC 25922 and S. typhimurium ATCC 13311 on Caco-2 cell line. In summaryItem Metadata only In vitro bactericidal efficacy of a new triple antibiotic paste formulation against Enterococcus faecalis biofilm(2022-01-01) Smittiset B.; Banomyong D.; Ruangsawasdi N.; Kaewprag J.; Mahidol UniversityThe aim of this study was to evaluate the bactericidal efficacy on Enterococcus faecalis biofilm of a novel, low-concentration triple-antibiotic paste (TAP-L2) in propylene glycol/carboxymethylcellulose vehicle (VEH-2), a ready-to-use prototype..., compared with two currently used products; a low-concentration (TAP-L1) and a high-concentration paste (TAP-H1) mixed in macrogol/propylene glycol vehicle (VEH-1). Sixty-two root canals were infected with E. faecalis biofilm, medicated with (a) TAP-L1, (bItem Metadata only Volatile anesthetic isoflurane exposure facilitates Enterococcus biofilm infection(2023-10-01) Maisat W.; Yuki K.; Mahidol UniversityEnterococcus faecalis (E. faecalis) is one of the major pathogenic bacteria responsible for surgical site infections. Biofilm infections are major hospital-acquired infections. Previous studies suggested that ions could regulate biofilm formation... in microbes. Volatile anesthetics, frequently administered in surgical setting, target ion channels. Here, we investigated the role of ion channels/transporters and volatile anesthetics in the biofilm formation by E. faecalis MMH594 strain and its ionItem Metadata only Isolation and characterization of Enterococcus faecalis isolate VT-H1: A highly efficient hydrogen-producing bacterium from palm oil mill effluent (POME)(2023-01-01) Woraruthai T.; Supawatkorn C.; Uthaipaisanwong P.; Kusonmano K.; Wongsurawat T.; Jenjaroenpun P.; Chaiyen P.; Wongnate T.; Mahidol Universityas Enterococcus faecalis isolate VT-H1 using hybrid sequencing technologies. Our investigation encompassed the growth of the isolate and its capacity for hydrogen and volatile fatty acid (VFA) production. The results revealed that optimal growth occurred when....0, outperforming previously reported E. faecalis strains by 5.84-fold. Kinetic analysis revealed favorable parameters for hydrogen production at 30 °C, with maximum hydrogen production (Hm = 2.63 mol-H2/mol-glucose), maximum hydrogen production rate (Rm = 0.03 mol